Red-emitting luciferases for bioluminescence reporter and imaging applications

BR Branchini, DM Ablamsky, AL Davis… - Analytical …, 2010 - Elsevier
The North American firefly Photinus pyralis luciferase, which emits yellow–green light (557nm),
has been adapted for a variety of applications, including gene reporter assays, whole-…

Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications

BR Branchini, DM Ablamsky, MH Murtiashaw… - Analytical …, 2007 - Elsevier
Light emission from the North American firefly Photinus pyralis, which emits yellow–green (557-nm)
light, is widely believed to be the most efficient bioluminescence system known, …

Red-and green-emitting firefly luciferase mutants for bioluminescent reporter applications

BR Branchini, TL Southworth, NF Khattak… - Analytical …, 2005 - Elsevier
Light emission from the North American firefly Photinus pyralis, which emits yellow–green (557-nm)
light, is widely believed to be the most efficient bioluminescence system known, …

An alternative mechanism of bioluminescence color determination in firefly luciferase

BR Branchini, TL Southworth, MH Murtiashaw… - Biochemistry, 2004 - ACS Publications
Beetle luciferases (including those of the firefly) use the same luciferin substrate to naturally
display light ranging in color from green (λ max ∼530 nm) to red (λ max ∼635 nm). In a …

Yellow-green and red firefly bioluminescence from 5, 5-dimethyloxyluciferin

BR Branchini, MH Murtiashaw, RA Magyar… - Journal of the …, 2002 - ACS Publications
Beetle luciferases (including those of the firefly) use the same luciferin substrate to naturally
display light ranging in color from green (λ max ∼ 530 nm) to red (λ max ∼ 635 nm). The …

Site-directed mutagenesis of histidine 245 in firefly luciferase: a proposed model of the active site

BR Branchini, RA Magyar, MH Murtiashaw… - Biochemistry, 1998 - ACS Publications
Firefly luciferase catalyzes the highly efficient emission of yellow-green light from substrate
luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We …

Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism

…, DM Fontaine, TL Southworth, BR Branchini… - Biochemistry, 2012 - ACS Publications
Beetle luciferases catalyze a two-step reaction that includes the initial adenylation of the
luciferin substrate, followed by an oxidative decarboxylation that ultimately produces light. …

The role of lysine 529, a conserved residue of the acyl-adenylate-forming enzyme superfamily, in firefly luciferase

BR Branchini, MH Murtiashaw, RA Magyar… - Biochemistry, 2000 - ACS Publications
Firefly luciferase catalyzes the highly efficient emission of yellow−green light from the
substrates luciferin, Mg-ATP, and oxygen in a two-step process. The enzyme first catalyzes the …

Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color

BR Branchini, RA Magyar, MH Murtiashaw… - Biochemistry, 1999 - ACS Publications
Under physiological conditions firefly luciferase catalyzes the highly efficient emission of
yellow−green light from the substrates luciferin, Mg-ATP, and oxygen. In nature, …

The role of active site residue arginine 218 in firefly luciferase bioluminescence

BR Branchini, RA Magyar, MH Murtiashaw… - Biochemistry, 2001 - ACS Publications
Firefly luciferase catalyzes the highly efficient emission of yellow−green light from substrate
firefly luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We …