[HTML][HTML] KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation

TH Tran, AH Chan, LC Young, L Bindu, C Neale… - Nature …, 2021 - nature.com
The first step of RAF activation involves binding to active RAS, resulting in the recruitment of
RAF to the plasma membrane. To understand the molecular details of RAS-RAF interaction, …

Structural basis of recognition of farnesylated and methylated KRAS4b by PDEδ

S Dharmaiah, L Bindu, TH Tran… - Proceedings of the …, 2016 - National Acad Sciences
Farnesylation and carboxymethylation of KRAS4b (Kirsten rat sarcoma isoform 4b) are
essential for its interaction with the plasma membrane where KRAS-mediated signaling events …

[PDF][PDF] Inhibition of Ras/Raf/MEK/ERK pathway signaling by a stress-induced phospho-regulatory circuit

DA Ritt, MT Abreu-Blanco, L Bindu, DE Durrant… - Molecular cell, 2016 - cell.com
Ras pathway signaling plays a critical role in cell growth control and is often upregulated in
human cancer. The Raf kinases selectively interact with GTP-bound Ras and are important …

Comparative analysis of 10 small molecules binding to carbonic anhydrase II by different investigators using Biacore technology

…, R Wilton, H Qiu, M Steukers, S Wang, L Bindu… - Analytical …, 2006 - Elsevier
In this benchmark study, 26 investigators were asked to characterize the kinetics and affinities
of 10 sulfonamide inhibitors binding to the enzyme carbonic anhydrase II using Biacore …

Synthesis and biological evaluation of indenoisoquinolines that inhibit both tyrosyl-DNA phosphodiesterase I (Tdp1) and topoisomerase I (Top1)

…, A Renaud, AG Stephen, LK Bindu… - Journal of medicinal …, 2013 - ACS Publications
Tyrosyl-DNA phosphodiesterase I (Tdp1) plays a key role in the repair of damaged DNA
resulting from the topoisomerase I (Top1) inhibitor camptothecin and a variety of other DNA-…

[HTML][HTML] Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions

…, D Esposito, M Abreu Blanco, P Alexander, L Bindu… - Scientific reports, 2015 - nature.com
Prenylated proteins play key roles in several human diseases including cancer, atherosclerosis
and Alzheimer’s disease. KRAS4b, which is frequently mutated in pancreatic, colon and …

[PDF][PDF] KRAS prenylation is required for bivalent binding with calmodulin in a nucleotide-independent manner

…, R Ghirlando, T Taylor, S Messing, TH Tran, L Bindu… - Biophysical journal, 2019 - cell.com
Deregulation of KRAS4b signaling pathway has been implicated in 30% of all cancers.
Membrane localization of KRAS4b is an essential step for the initiation of the downstream …

[HTML][HTML] Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane

B Lakshman, S Messing, EM Schmid… - Journal of Biological …, 2019 - ASBMB
The gene encoding the GTPase KRAS is frequently mutated in pancreatic, lung, and colorectal
cancers. The KRAS fraction in the plasma membrane (PM) correlates with activation of …

Membrane curvature sensing of the lipid-anchored K-Ras small GTPase

…, H Mu, F Jean-Francois, B Lakshman… - Life Science …, 2019 - life-science-alliance.org
Plasma membrane (PM) curvature defines cell shape and intracellular organelle morphologies
and is a fundamental cell property. Growth/proliferation is more stimulated in flatter cells …

The bacterial Ras/Rap1 site-specific endopeptidase RRSP cleaves Ras through an atypical mechanism to disrupt Ras-ERK signaling

…, A Herrera, PJ Woida, MB Kieffer, L Bindu… - Science …, 2018 - science.org
The Ras–extracellular signal–regulated kinase pathway is critical for controlling cell
proliferation, and its aberrant activation drives the growth of various cancers. Because many …