The T cell receptor/CD3 complex: a dynamic protein ensemble

H Clevers, B Alarcon, T Wileman… - Annual review of …, 1988 - annualreviews.org
… recognized the 14-kd CD3b chain (B. Alarcon, unpublished). The nature of the processing
event that reduces the apparent molecular weight of CD3-b to 14 kd remains unknown. The …

Internalization and intracellular fate of TCR-CD3 complexes

A Alcover, B Alarcon - Critical Reviews™ in Immunology, 2000 - dl.begellhouse.com
The number of surface TCR-CD3 complexes is maintained by an equilibrium between the
synthesis and secretion of new polypeptides. their internalization, recycling, and degradation. …

[HTML][HTML] Recruitment of Nck by CD3ϵ reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation

…, M Montoya, F Sánchez-Madrid, B Alarcón - Cell, 2002 - cell.com
… (B) CD3ϵ is the major binding partner for the SH3.1 domain. The indicated GST fusion
proteins were used for pd from 35 S-methionine Jurkat cell lysate. The position of the SH3.1 …

Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response

…, C Risco, JM Valpuesta, B Alarcón - The Journal of …, 2005 - rupress.org
… Valpuesta, Balbino Alarcón; Coexistence of multivalent and monovalent TCRs explains high
… Analogy between the TCR and the B cell antigen receptor (BCR) led to the hypothesis that …

Cell biology of T cell receptor expression and regulation

A Alcover, B Alarcón, V Di Bartolo - Annual review of …, 2018 - annualreviews.org
… several fine-tuned cellular processes that encompass (a) synthesis of the subunits and their
correct assembly and expression at the plasma membrane as a single functional complex, (b

PCR‐based procedures for detection and quantification of Staphylococcus aureus and their application in food

B Alarcon, B Vicedo, R Aznar - Journal of applied microbiology, 2006 - academic.oup.com
Aims: To evaluate the specificity of nuc targeted primers for PCR detection of Staphylococcus
aureus in different food matrices and to establish a RTQ‐PCR procedure suitable for the …

[PDF][PDF] Full activation of the T cell receptor requires both clustering and conformational changes at CD3

S Minguet, M Swamy, B Alarcón, IF Luescher… - Immunity, 2007 - cell.com
… alone was hardly active (Figure 4B), whereas both together induced strong phosphorylation,
similar to that evoked by stimulation with bivalent anti-CD3 (Figure 4B). Thus, induction of …

[PDF][PDF] T cell receptor internalization from the immunological synapse is mediated by TC21 and RhoG GTPase-dependent phagocytosis

…, DJ Irvine, B Huang, XR Bustelo, A Shaw, B Alarcón - Immunity, 2011 - cell.com
… , micrograph c), but they were not detected in the cSMAC (Figure 2B, micrograph b; quantification
in d). Finally, to prove the clathrin independence of TCR and TC21 cointernalization, a …

MTOC translocation modulates IS formation and controls sustained T cell signaling

…, M Gordón-Alonso, CH Sung, B Alarcón… - The Journal of cell …, 2008 - rupress.org
The translocation of the microtubule-organizing center (MTOC) toward the nascent immune
synapse (IS) is an early step in lymphocyte activation initiated by T cell receptor (TCR) …

[PDF][PDF] Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes

…, MT Rejas, JM Valpuesta, WWA Schamel, B Alarcon… - Immunity, 2011 - cell.com
… upon stimulation for 2 days with PHA and then expanded for 5 more days in medium containing
IL-2 and without PHA, were fixed and labeled with antibodies specific for CD3ε or TCR-b