INTRODUCTION The emergence of three lethal coronaviruses in <20 years and the urgency
of the COVID-19 pandemic have prompted efforts to develop new therapeutic strategies, …

Insulin-stimulated glucose uptake into muscle and adipose tissue is vital for maintaining
whole-body glucose homeostasis. Insulin promotes glucose uptake into these tissues by …

The failure of metabolic tissues to appropriately respond to insulin (“insulin resistance”) is
an early marker in the pathogenesis of type 2 diabetes. Protein phosphorylation is central to …

Secreted proteins fulfill a vast array of functions, including immunity, signaling, and extracellular
matrix remodeling. In the trans-Golgi network, proteins destined for constitutive secretion …

The in vitro oxygen microenvironment profoundly affects the capacity of cell cultures to
model physiological and pathophysiological states. Cell culture is often considered to be …

Insulin-induced GLUT4 translocation to the plasma membrane in muscle and adipocytes is
crucial for whole-body glucose homeostasis. Currently, GLUT4 trafficking assays rely on …

STX19 is an unusual Q a -SNARE as it lacks a C-terminal transmembrane domain. However,
it is efficiently targeted to post-Golgi membranes. Here, we set out to determine the …

Cell culture, the workhorse of biomedical research, is generally considered to be hyperoxic.
However, oxygen consumption by cells is underappreciated. High cellular respiration rates …

Constitutive secretion is predominantly measured by collecting the media from cells and
performing plate-based assays. This approach is particularly sensitive to changes in cell number…

The hormone leptin, primarily secreted by adipocytes, plays a crucial role in regulating
whole-body energy homeostasis. Homozygous loss-of-function mutations in the leptin gene (LEP) …