|
Status |
Public on Jun 21, 2018 |
Title |
RNA sequencing of ESC/iPSC-derived purified PAX6-GFP neural progenitors form control and Phelan-Mcdermid patients |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The goal of this study was to generated and transcriptionally analyze purified populations of Phelan-McDermid and control pluripotent stem cells-derived neural progenitors. This type of analysis allowed us to identify prevoisly unknown transcriptional phenotypic changes in patient neural progenitors which appear to be independent of functional synapses.
|
|
|
Overall design |
Patient and control pluripotent stem cells were targeted by CRISPR/Cas9 and a PAX6-GFP reporter plasmid. Correctly modified clones were selected and subsequently differentiated into PAX6 expressing neural progenitors.
|
|
|
Contributor(s) |
Jaenisch R, Roessler R |
Citation(s) |
30456368 |
Submission date |
Nov 30, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Reinhard Roessler |
Organization name |
Whitehead Institute
|
Street address |
455 Main street
|
City |
Cambridge |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA355605 |
SRA |
SRP094413 |