|
| Status |
Public on Sep 05, 2018 |
| Title |
CARM1 Methylates MED12 to Regulate its RNA Binding Ability |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The coactivator-associated arginine methyltransferase (CARM1) functions as a regulator for transcription, splicing and chromatin regulation by methylating a diverse array of substrates. In this study, we used CARM1 substrate antibodies to perform immunoprecipitation coupled with mass spectrometry (IP-MS) in MEFs and identified Mediator Subunit 12 (MED12) as a novel substrate for CARM1. ChIP-seq analysis using CARM1, MED12 and H3R17me2a (represents ‘CARM1 activity’) antibodies revealed that MED12 targeted by CARM1 is recruited to the EREs (estrogen-responsive elements). Additionally, RT-PCR studies showed that the MED12R1899K mutation disrupting the methylation site reduced the expression of ER-target genes. Thus, MED12 methylation targets it to ER-specific enhancers and positively modulates transcription of Estrogen-driven genes.
|
| |
|
| Overall design |
ChIP-seq of CARM1, MED12 and H3R17me2a in MCF7 cells
|
| |
|
| Contributor(s) |
Cheng D, Lu Y, Vemulapalli V, Bedford M |
| Citation(s) |
30456381 |
| Submission date |
Sep 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yue Lu |
| Organization name |
MD Anderson Cancer Center
|
| Department |
Epigenetics and Molecular Carcinogenesis
|
| Street address |
1808 Park Road 1C
|
| City |
Smithville |
| State/province |
TX |
| ZIP/Postal code |
78957 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA295184 |
| SRA |
SRP063514 |
Less...
More...