|
| Status |
Public on Sep 08, 2015 |
| Title |
Transformation of enriched mammary cell populations with Polyoma Middle T Antigen influences tumor subtype and metastatic potential |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Breast cancer is a multifaceted disease, exhibiting significant molecular, histological, and pathological diversity. Factors that impact this heterogeneity are poorly understood; however, transformation of distinct normal cell populations of the breast may generate different tumor phenotypes. Our previous study demonstrates that the polyomavirus middle T antigen (PyMT) oncogene can establish diverse tumor subtypes when broadly expressed within mouse mammary epithelial cells. Herein, we assess the molecular, histological, and metastatic outcomes from distinct mammary cell populations transformed with PyMT. By combining several methodologies, including lentiviral infection, cell sorting, and transplantation, we have characterized tumors arising from enriched populations of mammary epithelial cells. We have found that expression of PyMT within different cell populations influences tumor histology, molecular subtype, and metastatic potential.
|
| |
|
| Overall design |
32 samples, 1 from each of 32 mouse mammary tumors arising from transplanted mouse mamary epithelial cells (MMECs) transduced with PyMT-expressing lentivirus. MMECs were sorted into four different types prior to transplant: luminal CD133+ (8 samples), luminal CD133- (11 samples), stem (6 samples), and basal (7 samples). The background for the cell donor and transplant recipients mice was FVB/NJ obtained from Jackson Laboratories.
|
| |
|
| Contributor(s) |
Drobysheva D, Smith BA, Ekiz HA, Welm BE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 22, 2014 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Brett Milash |
| E-mail(s) |
brett.milash@utah.edu
|
| Organization name |
University of Utah
|
| Department |
Center for High Performance Computing
|
| Street address |
155 S. 1452 E. Room 414
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA271037 |
Less...
More...