|
| Status |
Public on Dec 23, 2014 |
| Title |
The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between cytoplasm and nuclei, and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific transcription and splicing of various sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may regulate mammalian muscle morphogenesis.
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| Overall design |
9 samples from Drosophila melanogaster were analyzed in duplicate: control dissected wildtype flight muscle at 30h APF, 72h APF and 0 day adult, jump muscle and whole leg from 1d adult and RNAi/mutant conditions for salm (1d flight muscle) and aret (30h, 72h and 1d flight muscle)
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| Contributor(s) |
Spletter ML, Schnorrer F, Gerlach D, Stark A, Yeroslaviz A, Habermann B |
| Citation(s) |
25532219, 34996845 |
| Submission date |
Nov 28, 2014 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Maria Lynn Spletter |
| E-mail(s) |
maria.spletter@umkc.edu
|
| Phone |
+19133135564
|
| Organization name |
University of Missouri Kansas City
|
| Department |
Biological and Biomedical Systems
|
| Lab |
RNA-dependent regulation during development processes
|
| Street address |
5009 Rockhill Rd
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA268841 |
| SRA |
SRP050336 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63707_DESeq2_analysisResults_AretIR0d_WTIFM0d_allGenes.txt.gz |
557.1 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_AretIR0d_salm_allGenes.txt.gz |
548.1 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_AretIR30h_WTIFM30h_allGenes.txt.gz |
509.7 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_AretIR72h_WTIFM72h_allGenes.txt.gz |
534.3 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_WTIFM0d_jump0d_allGenes.txt.gz |
543.4 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_WTIFM0d_leg0d_allGenes.txt.gz |
578.1 Kb |
(ftp)(http) |
TXT |
| GSE63707_DESeq2_analysisResults_WTIFM0d_salm_allGenes.txt.gz |
546.1 Kb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_AretIR_03d_vs_IFMWT_03d.txt.gz |
4.0 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_AretIR_03d_vs_salm_ko.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_AretIR_30h_vs_IFMWT_30h.txt.gz |
5.0 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_AretIR_72h_vs_IFMWT_72h.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_IFMWT_03d_vs_jump_0d.txt.gz |
3.3 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_IFMWT_03d_vs_leg_0d.txt.gz |
4.8 Mb |
(ftp)(http) |
TXT |
| GSE63707_DEXSeqResults_salm_ko_vs_IFMWT_03d.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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