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Status |
Public on Dec 02, 2005 |
Title |
Effects of neonatal dosing of SERMs on CD1 mouse uterine gene expression at 3 months |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Tamoxifen, a selective estrogen receptor modulator (SERM), is an effective treatment for breast cancers. In the CD-1 mouse model, neonatal oral dosing with tamoxifen leads to the development of adenomyosis. Both 4-hydroxyestradiol and tamoxifen can form DNA-reactive metabolites and may be involved in carcinogenesis of the uterus. After comparing the uterotrophic response of several SERMs the maximal uterotrophic doses of estradiol (100mg/kg) 4-hydroxyestradiol (385mg/kg) and tamoxifen (250mg/kg) were determined. Maximal uterotrophic doses were given orally to newborn CD-1 mice on days 1 – 4 after birth and gene and pathological changes examined in the uterus at 3 months after dosing. ERKOa knockout mice were dosed orally with tamoxifen (1mg/Kg) on days 1 – 4 after birth and uterine gene expression compared with CD-1 mice. Keywords: Comparison of SERMs at maximal uterotrophic doses
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Overall design |
Dosing groups: Estradiol (E2): 100mg/Kg 4-hydroxyestradiol (4OHE2): 385mg/Kg Tamoxifen : 250mg/Kg ERKO mice dosed with tamoxifen (1mg/Kg) 4 dosed animals and 4 controls for each treatment group. Uteri removed at 3 months after dosing and total RNA extracted. Controls were pooled. RNA labelling, hybridisation and analysis of fluorescence was carried out as described by Turton et al (2001). Cy3/Cy5 dye swap labelling was carried out on samples from each animal. Reference: Turton NJ et. al. (Oncogene (2001) 20, 1300-1306
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Citation missing |
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Submission date |
Nov 17, 2005 |
Last update date |
Mar 16, 2012 |
Contact name |
Jerry A Styles |
E-mail(s) |
jas12@le.ac.uk
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Phone |
441162231850
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Fax |
441162231855
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URL |
http://www.le.ac.uk/cm/rs/mrcmolend.html
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Organization name |
University of Leicester
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Department |
Cancer Studies and Molecular Medicine
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Lab |
MRC Molecular Endocrinology Laboratory
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Street address |
Robert Kilpatrick Building, Univ. Leicester
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City |
Leicester |
State/province |
Leicestershire |
ZIP/Postal code |
LE2 7LX |
Country |
United Kingdom |
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Platforms (2) |
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Samples (32)
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Relations |
BioProject |
PRJNA93731 |