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| Status |
Public on Jun 14, 2022 |
| Title |
ChIP-sequencing of PRMT1 proficient and deficient microglia |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP_sequencing analysis defined a novel role for the PRMT1 in microglia during normal and 0.2% cuprizone diet 5weeks
We then performed ChIP analysis using data obtained from ChIP-seq of microglia at two time points
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| Overall design |
Microglia ChIP profiles of 4-6 weeks-old wild-type (PRMT1Ifl/fl) and microglia specific knockout (PRMT1fl/fl; cx3r1cre-ert2) mice during normal and 0.2% cuprizone diet 5 weeks
Samples 1 to 10 (GSM6210097-GSM6210106) represent anti_H3K27Ac ChIP; while Samples 11 to 19 (GSM6210107-GSM6210115) are the inputs; they consisted in 1% aliquots of the samples taken post-sonication, but before IP.
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| |
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| Contributor(s) |
Lee J, Richard S, Gosselin D |
| Citation(s) |
35705491 |
| Submission date |
Jun 01, 2022 |
| Last update date |
Jul 14, 2022 |
| Contact name |
Stephane Richard |
| E-mail(s) |
stephane.richard@mcgill.ca
|
| Phone |
5143408260
|
| Organization name |
Lady Davis Institute- Jewish General Hospital
|
| Street address |
Lady Davis Institute, 3755 Cot
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (19)
|
| GSM6210097 |
PRMT1Ifl/fl microglia normal diet_rep1 |
| GSM6210098 |
PRMT1Ifl/fl microglia normal diet_rep2 |
| GSM6210099 |
PRMT1fl/fl; cx3r1cre-ert2 microglia normal diet_rep1 |
| GSM6210100 |
PRMT1fl/fl; cx3r1cre-ert2 microglia normal diet_rep2 |
| GSM6210101 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_low_rep1 |
| GSM6210102 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_low_rep2 |
| GSM6210103 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_high_rep1 |
| GSM6210104 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_high_rep2 |
| GSM6210105 |
PRMT1fl/fl; cx3r1cre-ert2 microglia CPZ diet_CD11c_low_rep1 |
| GSM6210106 |
PRMT1fl/fl; cx3r1cre-ert2 microglia CPZ diet_CD11c_low_rep2 |
| GSM6210107 |
PRMT1Ifl/fl microglia normal diet_Input_rep1 |
| GSM6210108 |
PRMT1Ifl/fl microglia normal diet_Input_rep2 |
| GSM6210109 |
PRMT1fl/fl; cx3r1cre-ert2 microglia normal diet_Input_rep1 |
| GSM6210110 |
PRMT1fl/fl; cx3r1cre-ert2 microglia normal diet_Input_rep2 |
| GSM6210111 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_low_Input_rep1 |
| GSM6210112 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_low_Input_rep2 |
| GSM6210113 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_high_Input_rep1 |
| GSM6210114 |
PRMT1Ifl/fl microglia CPZ diet_CD11c_high_Input_rep2 |
| GSM6210115 |
PRMT1fl/fl; cx3r1cre-ert2 microglia CPZ diet_CD11c_low_Input_rep2 |
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| Relations |
| BioProject |
PRJNA844360 |
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