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Status |
Public on Sep 29, 2021 |
Title |
Gut microbiota-derived short-chain fatty acids protect against the progression of endometriosis |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The goal of this study is to compare transcriptome profiling of n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) by RNA-sequencing Methods: The mRNA profiles of vehicle and n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) were generated by deep sequencing, in triplicate using Illumina NovaSeq-6000 sequencers with 2×150 paired-end reads. Basecalls and demultiplexing were performed with Illumina’s RTA version 1.9 and bcl2fastq2 software with a maximum of one mismatch in the indexing read. RNA-seq reads were then aligned to the Ensembl release 76 primary assembly with STAR version 2.5.1a. qRT–PCR validation was performed using TaqMan assays. Results: Using a 2.5-fold cutoff and Benjamini-Hochberg false discovery rate (FDR) of <0.01 threshold for inclusion, we identified 1,830 differentially expressed genes (DEGs) between Vehicle and n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc). Conclusions: Our study represents the first detailed analysis of n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc) transcriptomes, with biologic replicates, generated by RNA-seq technology.
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Overall design |
Transcriptome profiling of Vehicle and n-butyrate treated immortalized human endometriotic epithelial cells expression Luciferase (iHEECs/Luc)
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Contributor(s) |
Kommagani R, Chadchan SB, Popli P, Tycksen E |
Citation(s) |
34593556 |
Submission date |
Sep 20, 2021 |
Last update date |
Nov 03, 2021 |
Contact name |
Ramakrishna Kommagani |
E-mail(s) |
kommagani@wustl.edu
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Organization name |
Washington university School of Medicine in St. Louis.
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Department |
OB/GYN
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Lab |
Kommagani Lab.
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Street address |
425 S. Euclid Ave
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City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA764669 |
SRA |
SRP337858 |