|
| Status |
Public on Oct 05, 2021 |
| Title |
Gene expression analysis of yeast lacking Set4 under normal and stress conditions |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Genome-wide gene expression data were generated for wildtype and set4 mutant yeast under aerobic and hypoxic conditions to determine the contribution of Set4 to gene expression. Illumina-based sequencing was performed on strand-specific libraries prepared from mRNA purified from yeast grown in rich medium aerobically or under hypoxia. Reads were processed for qualiy control, mapped to the genome, and differential gene expression was determined based on log fold-change differences between set4 mutants and wildtype.
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| Overall design |
Three replicates each of wildtype and set4 mutant yeast grown in YPD medium, 30 degrees C either under aerobic or hypxic conditions are reported
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| |
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| Contributor(s) |
Jethmalani Y, Tran K, Ramos M, Garcia E, Negesse M, Park D, Green EM |
| Citation(s) |
34625508 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM124342 |
Lysine methylation at chromatin and cellular responses to stress |
UNIVERSITY OF MARYLAND, BALTIMORE COUNTY |
Erin Green |
|
| Submission date |
May 05, 2021 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Erin M Green |
| E-mail(s) |
egreen@umbc.edu
|
| Organization name |
UMBC
|
| Department |
Biological Sciences
|
| Street address |
1000 Hilltop Cir, Bs 325
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21250-0001 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA727533 |
| SRA |
SRP318564 |
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