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Status |
Public on Oct 01, 2021 |
Title |
Transcriptional adaptation to DNA replication stress induced by CCNE1 overexpression |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We overproduced human cyclin E from a stably-integrated inducible CCNE1 transgene in RPE1-hTERT cells over a period of weeks. CCNE1 overproduction induced replication stress, then slow proliferation, followed by adaptation to normal growth phenotype. We analyzed mRNA transcripts at key points of the timecourse: control, 2 days, 15-18 days, 39-51 days, and at a point several weeks after induction was withdrawn
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Overall design |
Retinal pigmented epithelial (RPE1-hTERT) mRNA profiles of CCNE1 overexpression in adaptation experiment over period of up to 51 days
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Contributor(s) |
Limas JC, Littlejohn AN, House AM, Kedziora KM, Aleman MM, Dominguez D, Cook JG |
Citation(s) |
35173014 |
Submission date |
Apr 11, 2021 |
Last update date |
Mar 15, 2022 |
Contact name |
Jeanette Cook |
E-mail(s) |
jean_cook@med.unc.edu
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Phone |
9198433867
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Organization name |
University of North Carolina at Chapel Hill
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Department |
Biochemistry and Biophysics
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Street address |
112 Founders Ridge Dr.
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City |
Chapel Hill |
State/province |
North Carolina |
ZIP/Postal code |
27517 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (15)
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GSM5235639 |
Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_1] |
GSM5235640 |
Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_2] |
GSM5235641 |
Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_3] |
GSM5235642 |
Acute population: Day 2 (“acute” CCNE1 overexpression, 48h) [t1_1] |
GSM5235643 |
Acute population: Day 2 (“acute” CCNE1 overexpression, 48h) [t1_2] |
GSM5235644 |
Acute population: Day 2 (“acute” CCNE1 overexpression, 48h) [t1_3] |
GSM5235645 |
“Crisis” population: Day 15 [t2_1] |
GSM5235646 |
“Crisis” population: Day 18 [t2_2] |
GSM5235647 |
“Crisis” population: Day 18 [t2_3] |
GSM5235648 |
Adapted population: Day 39 [t3_1] |
GSM5235649 |
Adapted population: Day 41 [t3_2] |
GSM5235650 |
Adapted population: Day 51 [t3_3] |
GSM5235651 |
Re-adapted population: After adapted 31 days with CCNE1 overexpression, allowed to grow w/x induction for 18 days [t4_1] |
GSM5235652 |
Re-adapted population: After adapted 42 days with CCNE1 overexpression, allowed to grow w/x induction for 46 days [t4_2] |
GSM5235653 |
Re-adapted population: After adapted 46 days with CCNE1 overexpression, allowed to grow w/x induction for 17 days [t4_3] |
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Relations |
BioProject |
PRJNA721187 |
SRA |
SRP314351 |