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| Status |
Public on Apr 15, 2021 |
| Title |
Single-cell RNA-sequencing of mouse islets exposed to proinflammatory cytokines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Exposure to proinflammatory cytokines is believed to contribute to pancreatic β-cells during diabetes development. While some cytokine-mediated changes in islet gene expression are known, the heterogeneity of the response is not well-understood. Following 6 hour treatment with interleukin-1 beta (IL-1β) and interferon-gamma (IFN-γ) alone or together, mouse islets were subjected to single-cell RNA-sequencing (scRNA-seq). Inducible nitric oxide synthase (iNOS) mRNA (Nos2), antiviral genes, and immune-associated genes were induced in a subset of β-cells in response to both cytokines, while IL-1β alone activated only antiviral genes. Subsets of α- and δ-cells expressed Nos2 and exhibited similar gene expression changes as β-cells, including induction of antiviral genes and repression of identity genes. Finally, cytokine-responsiveness was inversely correlated with expression of genes encoding heat shock proteins. Our findings show that all endocrine cell types respond to cytokines, IL-1β induces the expression of protective genes in β-cells, and cellular stress gene expression is associated with an inhibition in cytokine signaling.
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| Overall design |
Four samples of murine islets (no cytokine treatment, IL-1B only, IFNg only, IL-1B and IFNg exposure for 6 hours) were prepared using 10x Genomics Chromium platform
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| Contributor(s) |
Corbett J, Cui W, Stancill J |
| Citation(s) |
33883217 |
| Submission date |
Aug 13, 2020 |
| Last update date |
Jan 31, 2022 |
| Contact name |
Weiguo Cui |
| E-mail(s) |
wcui04@gmail.com
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| Phone |
312-503-0332
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| Organization name |
Northwestern University
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| Department |
Pathology
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| Lab |
Cui
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| Street address |
303 E Chicago Ave
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60654 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA656998 |
| SRA |
SRP277360 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156175_RAW.tar |
105.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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