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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 15, 2021 |
| Title |
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
The in vitro reconstitution of human germ-cell development provides a robust framework for clarifying key underlying mechanisms. Here, we explored transcription factors (TFs) that engender the germ-cell fate in their pluripotent precursors. Unexpectedly, SOX17, TFAP2C, and BLIMP1, which act under the BMP signaling and are indispensable for human primordial germ-cell-like cell (hPGCLC) specification, failed to induce hPGCLCs. In contrast, GATA3 or GATA2, immediate BMP effectors, combined with SOX17 and TFAP2C, generated hPGCLCs. GATA3/GATA2 knockouts dose-dependently impaired BMP-induced hPGCLC specification, whereas GATA3/GATA2 expression remained unaffected in SOX17, TFAP2C, or BLIMP1 knockouts. In cynomolgus monkeys, a key model for human development, GATA3, SOX17, and TFAP2C were co-expressed exclusively in early PGCs. Crucially, the TF-induced hPGCLCs acquired a hallmark of bona fide hPGCs to undergo epigenetic reprogramming and mature into oogonia/gonocytes in xenogeneic reconstituted ovaries. By uncovering a TF circuitry driving the germ line program, our study provides a paradigm for TF-based human gametogenesis.
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| Overall design |
Refer to individual Series
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| Citation(s) |
33608411 |
| Submission date |
Jul 19, 2020 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Yukihiro Yabuta |
| E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
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| Organization name |
Kyoto University, Graduate school of medicine
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| Department |
Anatomy and Cell Biology
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| Street address |
Yoshida-Konoe-cho, Sakyo-ku
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (184)
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| GSM4677257 |
Overexpression of TFAP2C, BLIMP1, clone_2; iPSC, MS151Y095 |
| GSM4677258 |
Overexpression of TFAP2C, BLIMP1, clone_2; day2, MS151Y098 |
| GSM4677259 |
Overexpression of TFAP2C, BLIMP1, clone_2; day4, MS151Y101 |
| GSM4677260 |
Overexpression of TFAP2C, BLIMP1, clone_2; iPSC, MS151Y107 |
| GSM4677261 |
Overexpression of TFAP2C, BLIMP1, clone_2; iMeLC, MS151Y110 |
| GSM4677262 |
Overexpression of TFAP2C, BLIMP1, clone_2; day1, MS151Y113 |
| GSM4677263 |
Overexpression of TFAP2C, BLIMP1, clone_2; day2, MS151Y116 |
| GSM4677264 |
Overexpression of TFAP2C, BLIMP1, clone_2; day4, MS151Y119 |
| GSM4677265 |
Overexpression of TFAP2C, BLIMP1, clone_2; iMeLC, MS151Y123 |
| GSM4677266 |
Overexpression of TFAP2C, BLIMP1, clone_2; day1, MS151Y126 |
| GSM4677267 |
Overexpression of TFAP2C, BLIMP1, clone_2; day2, MS152Y49 |
| GSM4677268 |
GATA2 KO, clone_1; iMeLC, MS168Y5 |
| GSM4677269 |
GATA2 KO, clone_6; iMeLC, MS168Y6 |
| GSM4677270 |
GATA2 KO, clone_12; iMeLC, MS168Y7 |
| GSM4677271 |
GATA2 KO, clone_1; day2, MS168Y15 |
| GSM4677272 |
GATA2 KO, clone_6; day2, MS168Y16 |
| GSM4677273 |
GATA2 KO, clone_12; day2, MS168Y17 |
| GSM4677274 |
GATA2 KO, clone_1; day4, MS168Y25 |
| GSM4677275 |
GATA2 KO, clone_6; day4, MS168Y26 |
| GSM4677276 |
GATA2 KO, clone_12; day4, MS168Y27 |
| GSM4677277 |
GATA3 KO, clone_30; iMeLC, MS168Y8 |
| GSM4677278 |
GATA3 KO, clone_30; day2, MS168Y18 |
| GSM4677279 |
GATA3 KO, clone_30; day4, MS168Y28 |
| GSM4677280 |
GATA3 KO, clone_17; iMeLC, MS168Y38 |
| GSM4677281 |
GATA3 KO, clone_18; iMeLC, MS168Y39 |
| GSM4677282 |
GATA3 KO, clone_40; iMeLC, MS168Y40 |
| GSM4677283 |
GATA3 KO, clone_17; day2, MS168Y44 |
| GSM4677284 |
GATA3 KO, clone_18; day2, MS168Y45 |
| GSM4677285 |
GATA3 KO, clone_40; day2, MS168Y46 |
| GSM4677286 |
GATA3 KO, clone_17; day4, MS168Y50 |
| GSM4677287 |
GATA3 KO, clone_18; day4, MS168Y51 |
| GSM4677288 |
GATA3 KO, clone_40; day4, MS168Y52 |
| GSM4677289 |
GATA3 KO, clone_17; day2, MS188Y102 |
| GSM4677290 |
GATA3 KO, clone_18; day2, MS188Y103 |
| GSM4677291 |
GATA3 KO, clone_30; day2, MS188Y104 |
| GSM4677292 |
GATA3 KO, clone_40; day2, MS188Y105 |
| GSM4677293 |
GATA3 KO, clone_17; day4, MS192Y108 |
| GSM4677294 |
GATA3 KO, clone_18; day4, MS188Y108 |
| GSM4677295 |
GATA3 KO, clone_30; day4, MS192Y109 |
| GSM4677296 |
GATA3 KO, clone_40; day4, MS188Y110 |
| GSM4677297 |
GATA3 KO, clone_17; day6, MS188Y112 |
| GSM4677298 |
GATA3 KO, clone_18; day6, MS188Y113 |
| GSM4677299 |
GATA3 KO, clone_30; day6, MS190Y114 |
| GSM4677300 |
GATA3 KO, clone_40; day6, MS190Y115 |
| GSM4677301 |
GATA3 KO, clone_17; day2, MS188Y117 |
| GSM4677302 |
GATA3 KO, clone_18; day2, MS188Y118 |
| GSM4677303 |
GATA3 KO, clone_30; day2, MS188Y119 |
| GSM4677304 |
GATA3 KO, clone_40; day2, MS188Y120 |
| GSM4677305 |
GATA3 KO, clone_17; day4, MS188Y122 |
| GSM4677306 |
GATA3 KO, clone_18; day4, MS188Y123 |
| GSM4677307 |
GATA3 KO, clone_30; day4, MS188Y124 |
| GSM4677308 |
GATA3 KO, clone_40; day4, MS188Y125 |
| GSM4677309 |
GATA3 KO, clone_17; day6, MS188Y127 |
| GSM4677310 |
GATA3 KO, clone_18; day6, MS188Y128 |
| GSM4677311 |
GATA3 KO, clone_30; day6, MS188Y129 |
| GSM4677312 |
GATA3 KO, clone_40; day6, MS188Y130 |
| GSM4677313 |
Ctrl of GATA2/3 KO, clone_BTAG; iMeLC, MS168Y9 |
| GSM4677314 |
Ctrl of GATA2/3 KO, clone_BTAG; day2, MS168Y19 |
| GSM4677315 |
Ctrl of GATA2/3 KO, clone_BTAG; day4, MS168Y29 |
| GSM4677316 |
Ctrl of GATA2/3 KO, clone_BTAG; day2, MS188Y106 |
| GSM4677317 |
Ctrl of GATA2/3 KO, clone_BTAG; day4, MS188Y111 |
| GSM4677318 |
Ctrl of GATA2/3 KO, clone_BTAG; day6, MS188Y116 |
| GSM4677319 |
Ctrl of GATA2/3 KO, clone_BTAG; day2, MS188Y121 |
| GSM4677320 |
Ctrl of GATA2/3 KO, clone_BTAG; day4, MS188Y126 |
| GSM4677321 |
Ctrl of GATA2/3 KO, clone_BTAG; day6, MS188Y131 |
| GSM4677322 |
Ctrl of Overexpression of SOX17, TFAP2C, GATA3, clone_BTAG; day6, MS188Y91 |
| GSM4677323 |
Ctrl of Overexpression of SOX17, TFAP2C, GATA3, clone_BTAG; day77, MS192Y107 |
| GSM4677324 |
Overexpression of SOX17, TFAP2C, clone_2; iPSC, MS151Y081 |
| GSM4677325 |
Overexpression of SOX17, TFAP2C, clone_11; iPSC, MS151Y082 |
| GSM4677326 |
Overexpression of SOX17, TFAP2C, clone_2; iMeLC, MS151Y083 |
| GSM4677327 |
Overexpression of SOX17, TFAP2C, clone_11; iMeLC, MS151Y084 |
| GSM4677328 |
Overexpression of SOX17, TFAP2C, clone_2; iMeLC, MS151Y085 |
| GSM4677329 |
Overexpression of SOX17, TFAP2C, clone_11; iMeLC, MS151Y086 |
| GSM4677330 |
Overexpression of SOX17, TFAP2C, clone_2; day2, MS151Y087 |
| GSM4677331 |
Overexpression of SOX17, TFAP2C, clone_2; day2, MS151Y088 |
| GSM4677332 |
Overexpression of SOX17, TFAP2C, clone_11; day2, MS151Y089 |
| GSM4677333 |
Overexpression of SOX17, TFAP2C, clone_11; day2, MS151Y090 |
| GSM4677334 |
Overexpression of SOX17, TFAP2C, clone_2; day2, MS151Y091 |
| GSM4677335 |
Overexpression of SOX17, TFAP2C, clone_11; day2, MS151Y092 |
| GSM4677336 |
Overexpression of SOX17, TFAP2C, clone_2; day2, MS151Y093 |
| GSM4677337 |
Overexpression of SOX17, TFAP2C, clone_11; day2, MS151Y094 |
| GSM4677338 |
Overexpression of SOX17, TFAP2C, clone_2; iMeLC, MS155Y57 |
| GSM4677339 |
Overexpression of SOX17, TFAP2C, clone_28; iMeLC, MS155Y58 |
| GSM4677340 |
Overexpression of SOX17, TFAP2C, clone_2; day1, MS155Y59 |
| GSM4677341 |
Overexpression of SOX17, TFAP2C, clone_28; day1, MS155Y60 |
| GSM4677342 |
Overexpression of SOX17, TFAP2C, clone_2; day2, MS155Y61 |
| GSM4677343 |
Overexpression of SOX17, TFAP2C, clone_28; day2, MS155Y62 |
| GSM4677344 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; iPSC, MS151Y096 |
| GSM4677345 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day2, MS151Y099 |
| GSM4677346 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y102 |
| GSM4677347 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y103 |
| GSM4677348 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y104 |
| GSM4677349 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y105 |
| GSM4677350 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y106 |
| GSM4677351 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; iPSC, MS151Y108 |
| GSM4677352 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; iMeLC, MS151Y111 |
| GSM4677353 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day1, MS151Y114 |
| GSM4677354 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day2, MS151Y117 |
| GSM4677355 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y120 |
| GSM4677356 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS151Y121 |
| GSM4677357 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; iMeLC, MS151Y124 |
| GSM4677358 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day1, MS151Y127 |
| GSM4677359 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day2, MS152Y50 |
| GSM4677360 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_4; day4, MS152Y54 |
| GSM4677361 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; iMeLC, MS152Y55 |
| GSM4677362 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; iMeLC, MS152Y56 |
| GSM4677363 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day1, MS152Y57 |
| GSM4677364 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day1, MS152Y58 |
| GSM4677365 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day4, MS152Y59 |
| GSM4677366 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day4, MS152Y60 |
| GSM4677367 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day4, MS152Y61 |
| GSM4677368 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day4, MS152Y62 |
| GSM4677369 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; iPSC, MS152Y63 |
| GSM4677370 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; iPSC, MS152Y64 |
| GSM4677371 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; iMeLC, MS152Y65 |
| GSM4677372 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; iMeLC, MS152Y66 |
| GSM4677373 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day1, MS152Y67 |
| GSM4677374 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day1, MS152Y68 |
| GSM4677375 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day1, MS152Y69 |
| GSM4677376 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day1, MS152Y70 |
| GSM4677377 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day1, MS152Y71 |
| GSM4677378 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day1, MS152Y72 |
| GSM4677379 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day2, MS152Y73 |
| GSM4677380 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day2, MS152Y74 |
| GSM4677381 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_22; day2, MS152Y75 |
| GSM4677382 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day2, MS152Y76 |
| GSM4677383 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day2, MS152Y77 |
| GSM4677384 |
Overexpression of SOX17, TFAP2C, BLIMP1, clone_28; day2, MS152Y78 |
| GSM4677385 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day2, MS168Y1 |
| GSM4677386 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day2, MS168Y2 |
| GSM4677387 |
Overexpression of SOX17, TFAP2C, GATA3, clone_11; day2, MS168Y3 |
| GSM4677388 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; iMeLC, MS168Y10 |
| GSM4677389 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; iMeLC, MS168Y11 |
| GSM4677390 |
Overexpression of SOX17, TFAP2C, GATA3, clone_11; iMeLC, MS168Y12 |
| GSM4677391 |
Overexpression of SOX17, TFAP2C, GATA3, clone_12; iMeLC, MS168Y13 |
| GSM4677392 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day2, MS168Y20 |
| GSM4677393 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day2, MS168Y21 |
| GSM4677394 |
Overexpression of SOX17, TFAP2C, GATA3, clone_11; day2, MS168Y22 |
| GSM4677395 |
Overexpression of SOX17, TFAP2C, GATA3, clone_12; day2, MS168Y23 |
| GSM4677396 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day4, MS168Y30 |
| GSM4677397 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day4, MS168Y31 |
| GSM4677398 |
Overexpression of SOX17, TFAP2C, GATA3, clone_11; day4, MS168Y32 |
| GSM4677399 |
Overexpression of SOX17, TFAP2C, GATA3, clone_12; day4, MS168Y33 |
| GSM4677400 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; iMeLC, MS168Y35 |
| GSM4677401 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; iMeLC, MS168Y36 |
| GSM4677402 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day2, MS168Y41 |
| GSM4677403 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day2, MS168Y42 |
| GSM4677404 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day4, MS168Y47 |
| GSM4677405 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day4, MS168Y48 |
| GSM4677406 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y89 |
| GSM4677407 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day6, MS188Y90 |
| GSM4677408 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y92 |
| GSM4677409 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day6, MS188Y93 |
| GSM4677410 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y94 |
| GSM4677411 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day6, MS188Y95 |
| GSM4677412 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y96 |
| GSM4677413 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day6, MS188Y97 |
| GSM4677414 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day8, MS188Y98 |
| GSM4677415 |
Overexpression of SOX17, TFAP2C, GATA3, clone_5; day8, MS188Y99 |
| GSM4677416 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y100 |
| GSM4677417 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS188Y101 |
| GSM4677418 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS192Y103 |
| GSM4677419 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day6, MS192Y104 |
| GSM4677420 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day77, MS192Y105 |
| GSM4677421 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day77, MS192Y106 |
| GSM4677422 |
Overexpression of SOX17, BLIMP1, clone_9; iPSC, MS151Y097 |
| GSM4677423 |
Overexpression of SOX17, BLIMP1, clone_9; day2, MS151Y100 |
| GSM4677424 |
Overexpression of SOX17, BLIMP1, clone_9; iPSC, MS151Y109 |
| GSM4677425 |
Overexpression of SOX17, BLIMP1, clone_9; iMeLC, MS151Y112 |
| GSM4677426 |
Overexpression of SOX17, BLIMP1, clone_9; day1, MS151Y115 |
| GSM4677427 |
Overexpression of SOX17, BLIMP1, clone_9; day2, MS151Y118 |
| GSM4677428 |
Overexpression of SOX17, BLIMP1, clone_9; day4, MS151Y122 |
| GSM4677429 |
Overexpression of SOX17, BLIMP1, clone_9; iMeLC, MS151Y125 |
| GSM4677430 |
Overexpression of SOX17, BLIMP1, clone_9; day1, MS151Y128 |
| GSM4677431 |
Overexpression of SOX17, BLIMP1, clone_9; day2, MS152Y52 |
| GSM4677432 |
Overexpression of SOX17, BLIMP1, clone_9; day2, MS152Y53 |
| GSM4677433 |
Overexpression of SOX17, GATA3, clone_2; day2, MS168Y4 |
| GSM4677434 |
Overexpression of SOX17, GATA3, clone_2; iMeLC, MS168Y14 |
| GSM4677435 |
Overexpression of SOX17, GATA3, clone_2; day2, MS168Y24 |
| GSM4677436 |
Overexpression of SOX17, GATA3, clone_2; day4, MS168Y34 |
| GSM4677437 |
Overexpression of SOX17, GATA3, clone_2; iMeLC, MS168Y37 |
| GSM4677438 |
Overexpression of SOX17, GATA3, clone_2; day2, MS168Y43 |
| GSM4677439 |
Overexpression of SOX17, GATA3, clone_2; day4, MS168Y49 |
| GSM4677539 |
Overexpression of SOX17, TFAP2C, GATA3, clone_1; day77 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE154688 |
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program [RNA-Seq] |
| GSE154690 |
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program [Bisulfite-Seq] |
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| Relations |
| BioProject |
PRJNA647202 |
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