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| Status |
Public on Jun 25, 2021 |
| Title |
Negative correlation of Pax3:Foxo1 expression at the single-cell-level with tumorigenic potential in rhabdomyosarcoma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In mouse Myf6Cre,Pax3:Foxo1,p53 rhabdomyosarcoma (RMS) cells, expression of Pax3:Foxo1 (P3F) is directed by the Pax3 promoter and coupled to an eYFP fluorescent marker.
YFPlow/P3Flow mouse RMS cells differentially expressed transcripts involved in extracellular matrix interaction, reorganized their actin cytoskeleton to promote adhesion/ migration and exhibited higher tumor-propagating capacity in limiting dilution analyses.
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| Overall design |
Total RNA was isolated from YFPlow/P3Flow, YFPhigh/P3Fhigh and unfractionated low-passage mouse RMS cells (U23674) and analyzed by Clariom S Assays, mouse arrays (Affymetrix 902931).
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| Contributor(s) |
Regina C, Andrieux G, Boerries M, Keller C, Hettmer S |
| Citation(s) |
34187933 |
| Submission date |
Jul 06, 2020 |
| Last update date |
Jul 06, 2021 |
| Contact name |
Geoffroy Andrieux |
| Organization name |
University clinics Freiburg
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| Street address |
Breisacherstr 153
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| City |
Freiburg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA644444 |
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