|
| Status |
Public on Jun 15, 2020 |
| Title |
Total poly(A)+ RNA sequencing |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Nuclear 3’ to 5’ nuclease RNA exosome plays a key role in quality control and processing of multiple protein-coding and non-coding transcripts made by RNA polymerase II. A mechanistic understanding of exosome function remains a challenge given the large number of RNA species and intervening RNA processing factors. Here we analysed changes in the poly(A)+ RNA proteins interactome provoked by mutations in three distinct subunits of the nuclear RNA exosome. Our data demonstrate a functional connection between Rrp6 and Mtl1 in controlling processing and levels of multiple protein-coding and non-coding transcripts. Furthermore, we show that exosome mutants accumulate components of U1 and U2 snRNPs and show depletion of NTC components from RNA suggesting that the stage prior to the activation of the spliceosome represents a critical quality control step. We have also identified potential new RNA binding factors involved in exosome regulation, including a zinc-finger protein called Mub1 that controls levels of selected transcripts encoding for proteins implicated in stress response. Collectively, our data have provided a global view of RNA metabolism alterations in exosome deficient cells and revealed RNA binding proteins that may act as novel exosome cofactors.
|
| |
|
| Overall design |
4 samples in triplicates
|
| |
|
| Contributor(s) |
Kilchert C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 23, 2020 |
| Last update date |
Jun 17, 2020 |
| Contact name |
Adrien Birot |
| E-mail(s) |
adrien.birot@bioch.ox.ac.uk
|
| Organization name |
University of Oxford
|
| Department |
Departement of Biochemistry
|
| Street address |
South Parks Rd
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA627618 |
| SRA |
SRP258063 |
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