|
| Status |
Public on Oct 20, 2021 |
| Title |
RNA-Seq during tumor cell plasticity and Platr18 overexpression/silencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The study aims: 1-to identify transcripts that are regulated during breast tumor cell plasticity induced by TGF-beta or by the silencing of PCBP1 expression and 2- to analyze the function of Platr18 lincRNA in breast cancer cells transcriptome reprogramming.
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| |
|
| Overall design |
NMuMG cells were treated with TGF-beta or with a PCBP1-shRNA to induce EMT. Cells over-expressing or silenced for Platr18 were also analyzed
|
| |
|
| Contributor(s) |
Grelet S, Fréreux C, Obellianne C, Noguchi K, Howley B, Dalton A, Howe P |
| Citation(s) |
34810279 |
| Submission date |
Mar 03, 2020 |
| Last update date |
Jan 07, 2022 |
| Contact name |
Simon Grelet |
| Organization name |
University of South Alabama - Mitchell Cancer Institute
|
| Street address |
1660 Spring Hill Ave - USA Drive North,
|
| City |
Mobile, AL |
| State/province |
Alabama |
| ZIP/Postal code |
36604 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (20)
|
| GSM4376561 |
NMuMG silenced for PCBP1 replicate 1 |
| GSM4376562 |
NMuMG silenced for PCBP1 replicate 2 |
| GSM4376563 |
NMuMG silenced for PCBP1 expressing scrambled shRNA replicate 1 |
| GSM4376564 |
NMuMG silenced for PCBP1 expressing scrambled shRNA replicate 2 |
| GSM4376565 |
NMuMG silenced for PCBP1 expressing platr18 shRNA replicate 1 |
| GSM4376566 |
NMuMG silenced for PCBP1 expressing platr18 shRNA replicate 2 |
| GSM4376567 |
NMuMG silenced for PCBP1 directed on the lungs replicate 1 |
| GSM4376568 |
NMuMG silenced for PCBP1 directed on the lungs replicate 2 |
| GSM4376569 |
NMuMG silenced for PCBP1 primary tumor replicate 1 |
| GSM4376570 |
NMuMG silenced for PCBP1 primary tumor replicate 2 |
| GSM4376571 |
NMuMG cells replicate 1 |
| GSM4376572 |
NMuMG cells replicate 2 |
| GSM4376573 |
NMuMG cells treated with TGFbeta replicate 1 |
| GSM4376574 |
NMuMG cells treated with TGFbeta replicate 2 |
| GSM4376575 |
NMuMG overexpressing Platr18 cl12 replicate 1 |
| GSM4376576 |
NMuMG overexpressing Platr18 cl12 replicate 2 |
| GSM4376577 |
NMuMG overexpressing Platr18 cl16 replicate 1 |
| GSM4376578 |
NMuMG overexpressing Platr18 cl16 replicate 2 |
| GSM4376579 |
NMuMG overexpressing empty vector replicate 1 |
| GSM4376580 |
NMuMG overexpressing empty vector replicate 2 |
|
| Relations |
| BioProject |
PRJNA609944 |
| SRA |
SRP251371 |
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