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| Status |
Public on Feb 03, 2020 |
| Title |
Gene expression profiles of control or PERK siRNA-transfected brown adipocyte during the differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The area of mitochondria in differentiated brown adipocytes is dramatically increased compared with that in brown pre-adipocytes. The ATP production is switched from the glycolysis pathway to the OXPHOS pathway during brown adipocyte differentiation. Endoplasmic reticulum (ER) is surrounded by mitochondria and the area of contact sites between mitochondria and the ER is increased. ER stress sensor PERK was phosphorylated during differentiation. To elucidate the mitochondria-ER crosstalk signaling mediated by PERK, RNA-sequencing analysis was performed using control siRNA- or PERK siRNA-transfected brown adipocytes.
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| Overall design |
Examination of gene expression in control or PERK siRNA-transfected brown adipocyte during the differentiation.
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| Contributor(s) |
Nishitoh H, Kato H, Tanimoto K |
| Citation(s) |
32029570 |
| Submission date |
Jun 12, 2019 |
| Last update date |
Feb 24, 2020 |
| Contact name |
Hideki Nishitoh |
| E-mail(s) |
nishitoh@med.miyazaki-u.ac.jp
|
| Organization name |
University of Miyazaki
|
| Department |
Department of Medical Sciences
|
| Lab |
Laboratory of Biochemistry and Molecular Biology
|
| Street address |
5200 Kihara, Kiyotake
|
| City |
Miyazaki City |
| State/province |
Miyazaki Prefecture |
| ZIP/Postal code |
889-1692 |
| Country |
Japan |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA548519 |
| SRA |
SRP201230 |
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