 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 25, 2019 |
| Title |
Cx3cr1-deficient microglia exhibit a premature aging transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA sequencing (RNA-Seq). In 2 mos mice, Cx3cr1 deletion resulted in the downregulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature.
|
| |
|
| Overall design |
RNAseq analysis of sorted microglia
|
| |
|
| Contributor(s) |
Gyoneva S, Hosur R, Challa R, Cullen P, Miao K, Allaire N, Roberts C |
| Citation(s) |
31792059 |
| Submission date |
May 28, 2019 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Stefka Gyoneva |
| E-mail(s) |
Stefka.gyoneva@biogen.com
|
| Phone |
617-914-6196
|
| Organization name |
Biogen, Inc
|
| Street address |
225 Binney St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (155)
|
|
| Relations |
| BioProject |
PRJNA545148 |
| SRA |
SRP199678 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE131869_CX3CR1_LPS_genes.estcount_table.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE131869_CX3CR1_LPS_genes.fpkm_table.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE131869_CX3CR1_LPS_genes.tpm_table.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE131869_CX3CR1_aging_genes.estcount_table.txt.gz |
3.7 Mb |
(ftp)(http) |
TXT |
| GSE131869_CX3CR1_aging_genes.fpkm_table.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
| GSE131869_CX3CR1_aging_genes.tpm_table.txt.gz |
3.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...