|
| Status |
Public on Feb 10, 2019 |
| Title |
Main constrains for RNAi induced by expressed long dsRNA in mouse cells |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Using a system expressing dsRNA molecules and luciferase reporters, we explored factors contributing to RNAi inefficiency in mouse embryonic stem cells (ESCs) and NIH3T3 fibroblasts that could explain previous contradictions concerning mammalian RNAi. Low and poorly processive Dicer activity combined with insufficiently abundant substrates appear to be the primary reason for RNAi inefficiency.
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| |
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| Overall design |
Bulk small RNA-seq of 8 NIH3T3 fibroblasts samples:
- 2 replicates transfected with pCag-EGFP Lin28IR
- 2 replicates transfected with pCag-EGFP MosIR
- 2 replicates transfected with pU6 Lin28IR
- 2 replicates transfected with pU6 MosIR
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| |
|
| Contributor(s) |
Svoboda P, Malik R, Horvat F |
| Citation(s) |
30808654 |
| BioProject |
PRJNA521069 |
| Submission date |
Feb 08, 2019 |
| Last update date |
May 13, 2019 |
| Contact name |
Filip Horvat |
| E-mail(s) |
filip.horvat@img.cas.cz
|
| Organization name |
Institute of Molecular Genetics of the ASCR
|
| Lab |
Laboratory of Epigenetic Regulations
|
| Street address |
Videnska 1083
|
| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
|
| GSM3596393 |
pCag-EGFP Lin28IR transfected NIH3T3 cells, replicate 1 |
| GSM3596394 |
pCag-EGFP Lin28IR transfected NIH3T3 cells, replicate 2 |
| GSM3596395 |
pCag-EGFP MosIR transfected NIH3T3 cells, replicate 1 |
| GSM3596396 |
pCag-EGFP MosIR transfected NIH3T3 cells, replicate 2 |
| GSM3596397 |
pU6 Lin28IR transfected NIH3T3 cells, replicate 1 |
| GSM3596398 |
pU6 Lin28IR transfected NIH3T3 cells, replicate 2 |
| GSM3596399 |
pU6 MosIR transfected NIH3T3 cells, replicate 1 |
| GSM3596400 |
pU6 MosIR transfected NIH3T3 cells, replicate 2 |
|
| Relations |
| SRA |
SRP183809 |
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