Abstract
Full-length cDNAs are essential for functional analysis of plant genes. We constructed high-content, full-length cDNA libraries from Arabidopsis thaliana plants based on chemical introduction of a biotin group into the diol residue of the CAP structure of eukaryotic mRNA, followed by RNase I treatment, to select full-length cDNA. More than 90% of the total clones obtained were of full length; recombinant clones were obtained with high efficiency (2.2 x 10(6)/9 micrograms starting mRNA). Sequence analysis of 111 randomly picked clones indicated that 32 isolated cDNA groups were derived from novel genes in the A. thaliana genome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Biotinylation
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Cloning, Molecular / methods*
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DNA, Complementary*
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DNA, Plant*
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Genomic Library
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RNA Caps*
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RNA, Messenger / genetics
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RNA, Plant / genetics
Substances
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DNA, Complementary
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DNA, Plant
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RNA Caps
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RNA, Messenger
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RNA, Plant
Associated data
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GENBANK/AB015096
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GENBANK/AB015097
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GENBANK/AB015098
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GENBANK/AB015099
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GENBANK/AB015100
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GENBANK/AB015101
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GENBANK/AB015102
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GENBANK/AB015103
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GENBANK/AB015104
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GENBANK/AB015105
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GENBANK/AB015106
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GENBANK/AB015107
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GENBANK/AB015108
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GENBANK/AB015109
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GENBANK/AB015110
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GENBANK/AB015111
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GENBANK/AB015112
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GENBANK/AB015113
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GENBANK/AB015114
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GENBANK/AB015115
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GENBANK/AB015116
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GENBANK/AB015117
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GENBANK/AB015118
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GENBANK/AB015119
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GENBANK/AB015120
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GENBANK/AB015121
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GENBANK/AB015122
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GENBANK/AB015123
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GENBANK/AB015124
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GENBANK/AB015125