Abstract
Mannose 6-phosphate receptors (MPRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. We have identified a 47 kDa protein (TIP47) that binds selectively to the cytoplasmic domains of cation-independent and cation-dependent MPRs. TIP47 is present in cytosol and on endosomes and is required for MPR transport from endosomes to the trans-Golgi network in vitro and in vivo. TIP47 recognizes a phenylalanine/tryptophan signal in the tail of the cation-dependent MPR that is essential for its proper sorting within the endosomal pathway. These data suggest that TIP47 binds MPR cytoplasmic domains and facilitates their collection into transport vesicles destined for the Golgi.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Animals
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Base Sequence
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Biological Transport
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COS Cells / chemistry
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Cattle
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Cell Membrane / chemistry
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Cloning, Molecular
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Cytoplasm / metabolism
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Cytosol / chemistry
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Endosomes / chemistry
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Epithelial Cells / chemistry
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Golgi Apparatus / metabolism
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Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
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Humans
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Intracellular Signaling Peptides and Proteins*
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Molecular Sequence Data
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Perilipin-3
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Pregnancy Proteins*
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Receptor, IGF Type 2 / metabolism*
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Recombinant Fusion Proteins / metabolism
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Vesicular Transport Proteins
Substances
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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PLIN3 protein, human
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Perilipin-3
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Pregnancy Proteins
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Receptor, IGF Type 2
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Recombinant Fusion Proteins
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Vesicular Transport Proteins
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Guanosine 5'-O-(3-Thiotriphosphate)