The Schizosaccharomyces pombe cdc6 gene encodes the catalytic subunit of DNA polymerase delta

Y Iino; M Yamamoto

doi:10.1007/s004380050395

The Schizosaccharomyces pombe cdc6 gene encodes the catalytic subunit of DNA polymerase delta

Mol Gen Genet. 1997 Mar 18;254(1):93-7. doi: 10.1007/s004380050395.

Authors

Y Iino¹, M Yamamoto

Affiliation

¹ Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Japan.

PMID: 9108295
DOI: 10.1007/s004380050395

Abstract

The cdc6 mutants of Schizosaccharomyces pombe have been classified as being defective in progression through the G2 phase of the cell cycle. We cloned an S. pombe gene that could complement the temperature-sensitive growth of the cdc6-23 mutant. Unexpectedly, the cloned gene was allelic to pol3, which encodes the catalytic subunit of DNA polymerase delta. Integration mapping confirmed that ccd6 and pol3 are identical. The cdc6-23 mutant carries one amino acid substitution in the conserved N3 region of Pol3.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Cycle Proteins / genetics*
Cell Cycle*
Cloning, Molecular
DNA Polymerase III
DNA-Directed DNA Polymerase / genetics*
Fungal Proteins / genetics*
Genes, Fungal*
Molecular Sequence Data
Saccharomyces cerevisiae Proteins*
Schizosaccharomyces / enzymology
Schizosaccharomyces / genetics*
Schizosaccharomyces pombe Proteins*

Substances

CDC6 protein, S cerevisiae
Cell Cycle Proteins
Fungal Proteins
Saccharomyces cerevisiae Proteins
Schizosaccharomyces pombe Proteins
cdc6 protein, S pombe
DNA Polymerase III
DNA-Directed DNA Polymerase