Abstract
The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucleating unit which can cap the minus ends of microtubules in vitro.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Chromatography, Affinity
-
Microscopy, Electron
-
Microtubules / metabolism*
-
Microtubules / ultrastructure
-
Molecular Sequence Data
-
Oocytes / chemistry
-
Protein Conformation
-
Protein Structure, Secondary
-
Tubulin / chemistry*
-
Tubulin / isolation & purification
-
Tubulin / metabolism
-
Tubulin / ultrastructure
-
Xenopus