Abstract
Transfection of embryo fibroblasts by a human ras oncogene does not convert them into tumour cells unless the fibroblasts are established and immortalized before transfection. The embryo fibroblasts become tumorigenic if a second oncogene such as a viral or cellular myc gene or the gene for the polyoma large-T antigen is introduced together with the ras gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Viral / genetics
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Antigens, Viral, Tumor
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Cell Line
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Cell Transformation, Viral*
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Cells, Cultured
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Fibroblasts
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Gene Expression Regulation
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Genes, Viral
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Genetic Complementation Test
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Mice
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Oncogenes*
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Phenotype
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Polyomavirus / genetics
Substances
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Antigens, Viral
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Antigens, Viral, Tumor