Bone-Marrow-Resident NK Cells Prime Monocytes for Regulatory Function during Infection

Michael H Askenase; Seong-Ji Han; Allyson L Byrd; Denise Morais da Fonseca; Nicolas Bouladoux; Christoph Wilhelm; Joanne E Konkel; Timothy W Hand; Norinne Lacerda-Queiroz; Xin-zhuan Su; Giorgio Trinchieri; John R Grainger; Yasmine Belkaid

doi:10.1016/j.immuni.2015.05.011

Bone-Marrow-Resident NK Cells Prime Monocytes for Regulatory Function during Infection

Immunity. 2015 Jun 16;42(6):1130-42. doi: 10.1016/j.immuni.2015.05.011. Epub 2015 Jun 9.

Affiliations

¹ Program in Barrier Immunity and Repair, Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA; Immunology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA.
² Program in Barrier Immunity and Repair, Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
³ Program in Barrier Immunity and Repair, Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA; Translational and Functional Genomics Branch, National Human Genome Research Institute, NIH, Bethesda, MD 20892, USA.
⁴ Manchester Collaborative Centre for Inflammation Research (MCCIR), University of Manchester, Manchester M13 9NT, UK; Faculty of Life Sciences, University of Manchester, Manchester M13 9NT, UK.
⁵ Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
⁶ Laboratory of Experimental Immunology, Head, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
⁷ Program in Barrier Immunity and Repair, Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA; Manchester Collaborative Centre for Inflammation Research (MCCIR), University of Manchester, Manchester M13 9NT, UK; Faculty of Life Sciences, University of Manchester, Manchester M13 9NT, UK. Electronic address: john.grainger-2@manchester.ac.uk.
⁸ Program in Barrier Immunity and Repair, Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA. Electronic address: ybelkaid@niaid.nih.gov.

Abstract

Tissue-infiltrating Ly6C(hi) monocytes play diverse roles in immunity, ranging from pathogen killing to immune regulation. How and where this diversity of function is imposed remains poorly understood. Here we show that during acute gastrointestinal infection, priming of monocytes for regulatory function preceded systemic inflammation and was initiated prior to bone marrow egress. Notably, natural killer (NK) cell-derived IFN-γ promoted a regulatory program in monocyte progenitors during development. Early bone marrow NK cell activation was controlled by systemic interleukin-12 (IL-12) produced by Batf3-dependent dendritic cells (DCs) in the mucosal-associated lymphoid tissue (MALT). This work challenges the paradigm that monocyte function is dominantly imposed by local signals after tissue recruitment, and instead proposes a sequential model of differentiation in which monocytes are pre-emptively educated during development in the bone marrow to promote their tissue-specific function.

Publication types

Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Ly / metabolism
Basic-Leucine Zipper Transcription Factors / genetics
Basic-Leucine Zipper Transcription Factors / metabolism
Bone Marrow Cells / immunology*
Bone Marrow Cells / parasitology
Cell Differentiation
Cells, Cultured
Dendritic Cells / immunology*
Interferon-gamma / metabolism
Interleukin-12 / genetics
Interleukin-12 / metabolism
Intestinal Mucosa / immunology*
Intestinal Mucosa / parasitology
Killer Cells, Natural / immunology*
Killer Cells, Natural / parasitology
Leukocytes, Mononuclear / immunology*
Leukocytes, Mononuclear / parasitology
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Knockout
Models, Immunological
Organ Specificity / immunology
Repressor Proteins / genetics
Repressor Proteins / metabolism
Toxoplasma / immunology*
Toxoplasmosis / immunology*

Substances

Antigens, Ly
Basic-Leucine Zipper Transcription Factors
Ly-6C antigen, mouse
Repressor Proteins
SNFT protein, mouse
Interleukin-12
Interferon-gamma

Bone-Marrow-Resident NK Cells Prime Monocytes for Regulatory Function during Infection

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

Grants and funding