Abstract
miR-155 plays a crucial role in proinflammatory activation. This study was carried out to assess the association of abnormal expression of miR-155 in peripheral blood of patients with Rheumatoid arthritis with the expression of TNF-α and IL-1β. Release of TNF-α and IL-1β, and expression of miR-155 were determined in RA peripheral blood or peripheral blood macrophages, followed by correlation analysis of the cytokines release and miR-155 expression. Furthermore, in vitro studies indicate that miR-155 inhibited the expression of SOCS1. Our results suggest that there is a correlation between the high-level expression of miR-155 and the enhanced expression of TNF-α and IL-1β. miR-155 targets and suppresses the expression of SOCS1, and the decrease of SOCS1 may lead to the upregulation of TNF-α and IL-1β.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3' Untranslated Regions
-
Adult
-
Arthritis, Rheumatoid / genetics
-
Arthritis, Rheumatoid / metabolism
-
Arthritis, Rheumatoid / pathology
-
Base Sequence
-
Female
-
Humans
-
Interleukin-1beta / genetics
-
Interleukin-1beta / metabolism*
-
Leukocytes, Mononuclear / metabolism*
-
Lipopolysaccharides / toxicity
-
Male
-
MicroRNAs / antagonists & inhibitors
-
MicroRNAs / metabolism*
-
Middle Aged
-
Oligonucleotides, Antisense / metabolism
-
RNA, Messenger / metabolism
-
Suppressor of Cytokine Signaling 1 Protein
-
Suppressor of Cytokine Signaling Proteins / antagonists & inhibitors*
-
Suppressor of Cytokine Signaling Proteins / genetics
-
Suppressor of Cytokine Signaling Proteins / metabolism
-
Tumor Necrosis Factor-alpha / genetics
-
Tumor Necrosis Factor-alpha / metabolism*
-
Up-Regulation / drug effects
-
Young Adult
Substances
-
3' Untranslated Regions
-
Interleukin-1beta
-
Lipopolysaccharides
-
MIRN155 microRNA, human
-
MicroRNAs
-
Oligonucleotides, Antisense
-
RNA, Messenger
-
SOCS1 protein, human
-
Suppressor of Cytokine Signaling 1 Protein
-
Suppressor of Cytokine Signaling Proteins
-
Tumor Necrosis Factor-alpha