SNAP-25 regulates spine formation through postsynaptic binding to p140Cap

Romana Tomasoni; Daniele Repetto; Raffaella Morini; Chiara Elia; Fabrizio Gardoni; Monica Di Luca; Emilia Turco; Paola Defilippi; Michela Matteoli

doi:10.1038/ncomms3136

SNAP-25 regulates spine formation through postsynaptic binding to p140Cap

Nat Commun. 2013:4:2136. doi: 10.1038/ncomms3136.

Authors

Romana Tomasoni¹, Daniele Repetto, Raffaella Morini, Chiara Elia, Fabrizio Gardoni, Monica Di Luca, Emilia Turco, Paola Defilippi, Michela Matteoli

Affiliation

¹ Department of Biotechnology and Translational Medicine, University of Milan, V Vanvitelli 32, Milano 20129, Italy.

PMID: 23868368
DOI: 10.1038/ncomms3136

Abstract

Synaptosomal-associated protein of 25 kDa (SNAP-25) is a member of the Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) protein family, required for exocytosis of synaptic vesicles and regulation of diverse ion channels. Here, we show that acute reduction of SNAP-25 expression leads to an immature phenotype of dendritic spines that are, consistently, less functional. Conversely, over-expression of SNAP-25 results in an increase in the density of mature, Postsynaptic Density protein 95 (PSD-95)-positive spines. The regulation of spine morphogenesis by SNAP-25 depends on the protein's ability to bind both the plasma membrane and the adaptor protein p140Cap, a key protein regulating actin cytoskeleton and spine formation. We propose that SNAP-25 allows the organization of the molecular apparatus needed for spine formation by recruiting and stabilizing p140Cap.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / genetics
Actin Cytoskeleton / metabolism
Adaptor Proteins, Vesicular Transport / genetics*
Adaptor Proteins, Vesicular Transport / metabolism
Animals
Dendritic Spines / metabolism*
Dendritic Spines / ultrastructure
Disks Large Homolog 4 Protein
Embryo, Mammalian
Gene Expression Regulation, Developmental*
HeLa Cells
Hippocampus / cytology
Hippocampus / embryology
Hippocampus / metabolism*
Humans
Intracellular Signaling Peptides and Proteins / genetics*
Intracellular Signaling Peptides and Proteins / metabolism
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Primary Cell Culture
Protein Binding
Protein Stability
Rats
Signal Transduction
Synaptosomal-Associated Protein 25 / genetics*
Synaptosomal-Associated Protein 25 / metabolism

Substances

Adaptor Proteins, Vesicular Transport
Disks Large Homolog 4 Protein
Dlg4 protein, rat
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Snap25 protein, rat
Srcin1 protein, rat
Synaptosomal-Associated Protein 25