Abstract
SOX2 was reported to promote metastasis in various tumor tissues; however the underlying mechanisms remain elusive. Here, we disclosed that SOX2 improves metastasis of breast and prostate cancer cells by promoting epithelial-to-mesenchymal transition (EMT) through WNT/β-catenin, but not TGF-β or Snail1 signaling. Dual luciferase assay and chromatin immunoprecipitation revealed activation and binding of SOX2 on promoter region of β-catenin. In addition, SOX2 affects the protein expression levels of DKK3, DVL1 and DVL3, which are regulators or downstream molecules of WNT signaling. Taken together, our findings demonstrated β-catenin as one of vital downstream molecules that mediate the EMT induced by SOX2.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / biosynthesis
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Animals
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Cell Line, Tumor
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Chemokines
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Dishevelled Proteins
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Epithelial-Mesenchymal Transition
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Female
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Humans
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Intercellular Signaling Peptides and Proteins / biosynthesis
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MCF-7 Cells
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Male
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Mice
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Mice, Inbred NOD
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Mice, SCID
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Phosphoproteins / biosynthesis
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Prostatic Neoplasms / metabolism*
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Prostatic Neoplasms / pathology
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SOXB1 Transcription Factors / genetics
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SOXB1 Transcription Factors / metabolism*
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Signal Transduction
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Wnt Proteins / metabolism*
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Xenograft Model Antitumor Assays
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beta Catenin / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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CTNNB1 protein, human
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Chemokines
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DKK3 protein, human
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DVL1 protein, human
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DVL3 protein, human
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Dishevelled Proteins
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Dvl1 protein, mouse
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Dvl3 protein, mouse
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Intercellular Signaling Peptides and Proteins
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Phosphoproteins
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SOX2 protein, human
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SOXB1 Transcription Factors
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Wnt Proteins
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beta Catenin