Abstract
In malaria vaccine research, one of the major obstacles has been the difficulty of expressing recombinant malarial proteins and it is mainly due to the lack of an efficient methodology for the synthesis of sufficient quantity of quality proteins. We demonstrate that the wheat germ cell-free protein synthesis system can be applied for the successful production of leading malaria vaccine candidate antigens and, thus, prove that it may be a key tool for malaria vaccine research.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antigens, Protozoan / biosynthesis*
-
Antigens, Protozoan / genetics
-
Antigens, Protozoan / immunology
-
Antigens, Protozoan / isolation & purification
-
Cell-Free System
-
Chromatography, Affinity
-
Female
-
Glycosylation
-
Humans
-
Malaria Vaccines / biosynthesis*
-
Malaria Vaccines / genetics
-
Malaria Vaccines / immunology
-
Malaria Vaccines / isolation & purification
-
Mice
-
Mice, Inbred BALB C
-
Microscopy, Confocal
-
Microscopy, Fluorescence
-
Plasmodium falciparum / genetics
-
Plasmodium falciparum / immunology*
-
Protein Biosynthesis*
-
Protein Engineering / methods*
-
Protein Folding
-
Protein Processing, Post-Translational
-
Protozoan Proteins / biosynthesis*
-
Protozoan Proteins / genetics
-
Protozoan Proteins / immunology
-
Protozoan Proteins / isolation & purification
-
Seeds / metabolism
-
Transcription, Genetic
-
Triticum / embryology
-
Triticum / genetics
-
Triticum / metabolism*
Substances
-
Antigens, Protozoan
-
Malaria Vaccines
-
Pfs25 protein, Plasmodium falciparum
-
Protozoan Proteins