Abstract
Plasmid pGL4W15-96 was constructed from the pGL4W74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed J15 promoter with the objective of confirming the role of J15 sequence as a promoter for Leptospira, which restored the transcription of the gene of resistance to kanamycin in Escherichia coli and Leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Bacterial Proteins*
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Drug Resistance, Bacterial / genetics*
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Escherichia coli / drug effects
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Kanamycin / pharmacology*
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Leptospira interrogans / drug effects*
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Leptospira interrogans / genetics*
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Leptospira interrogans / metabolism
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Lipopolysaccharides / biosynthesis*
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O Antigens
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Plasmids / genetics*
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Promoter Regions, Genetic / genetics
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Transcription, Genetic*
Substances
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Bacterial Proteins
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Lipopolysaccharides
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O Antigens
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rfb protein, Bacteria
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Kanamycin