Abstract
The hypothesis that the cytosolic RNase inhibitor (cRI) has a role in the protection of the cellular redox homeostasis was investigated testing the effects of oxidants and anti-oxidants on normal, primary endothelial HUVE cells, and malignant HeLa cells, before and after their engineering into cRI-deprived cells. We found that cRI plays an important, possibly a key, physiological role in the protection of cells from redox stress, as demonstrated by decreased GSH levels as well as increased oxidant-induced DNA damage in cRI deprived cells.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antioxidants / metabolism
-
Carrier Proteins / antagonists & inhibitors
-
Carrier Proteins / genetics
-
Carrier Proteins / metabolism*
-
Cells, Cultured
-
Endothelial Cells / drug effects
-
Endothelial Cells / metabolism
-
Enzyme Inhibitors / metabolism*
-
Glutathione / metabolism
-
HeLa Cells
-
Homeostasis
-
Humans
-
Oxidation-Reduction
-
RNA Interference
-
RNA, Small Interfering / genetics
-
Ribonucleases / antagonists & inhibitors*
Substances
-
Antioxidants
-
Carrier Proteins
-
Enzyme Inhibitors
-
RNA, Small Interfering
-
RNH1 protein, human
-
Ribonucleases
-
Glutathione