Abstract
The estrogen receptor is the master transcriptional regulator of breast cancer phenotype and the archetype of a molecular therapeutic target. We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. Combining this unique resource with gene expression data demonstrates distinct temporal mechanisms of estrogen-mediated gene regulation, particularly in the case of estrogen-suppressed genes. Furthermore, this resource has allowed the identification of cis-regulatory sites in previously unexplored regions of the genome and the cooperating transcription factors underlying estrogen signaling in breast cancer.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing / metabolism
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Adenocarcinoma / genetics
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Breast Neoplasms / genetics
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Cells, Cultured
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Chromosome Mapping / methods
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Conserved Sequence
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DNA-Binding Proteins / metabolism
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Down-Regulation
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Gene Expression
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Gene Expression Regulation
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Genome, Human*
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Humans
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Microarray Analysis / methods
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Nuclear Proteins / metabolism
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Nuclear Receptor Interacting Protein 1
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Receptors, Estrogen / metabolism*
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Response Elements* / physiology
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Transcription Factors / physiology
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Transcription Initiation Site
Substances
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Adaptor Proteins, Signal Transducing
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DNA-Binding Proteins
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Nuclear Proteins
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Nuclear Receptor Interacting Protein 1
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Receptors, Estrogen
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Transcription Factors