Abstract
Condensins are essential protein complexes critical for mitotic chromosome organization. Little is known about the function of condensins during interphase, particularly in mammalian cells. Here we report the interphase-specific interaction between condensin I and the DNA nick-sensor poly(ADP-ribose) polymerase 1 (PARP-1). We show that the association between condensin I, PARP-1, and the base excision repair (BER) factor XRCC1 increases dramatically upon single-strand break damage (SSB) induction. Damage-specific association of condensin I with the BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/epsilon was also observed, suggesting that condensin I is recruited to interact with BER factors at damage sites. Consistent with this, DNA damage rapidly stimulates the chromatin association of PARP-1, condensin I, and XRCC1. Furthermore, depletion of condensin in vivo compromises SSB but not double-strand break (DSB) repair. Our results identify a SSB-specific response of condensin I through PARP-1 and demonstrate a role for condensin in SSB repair.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine Triphosphatases / metabolism
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Adenosine Triphosphatases / physiology*
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Animals
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Animals, Genetically Modified
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Carrier Proteins
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Cell Cycle Proteins
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Cell Line
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Chickens / genetics
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA Damage*
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DNA Repair*
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DNA, Single-Stranded
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DNA-Binding Proteins / metabolism*
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DNA-Binding Proteins / physiology*
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HeLa Cells
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Humans
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Interphase
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Mass Spectrometry
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Mice / genetics
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Mice, Knockout
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Models, Biological
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Multiprotein Complexes / metabolism
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Multiprotein Complexes / physiology*
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Nuclear Proteins
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Poly(ADP-ribose) Polymerases / genetics*
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Poly(ADP-ribose) Polymerases / metabolism
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Poly(ADP-ribose) Polymerases / physiology
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Transfection
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X-ray Repair Cross Complementing Protein 1
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Xenopus Proteins
Substances
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Carrier Proteins
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA, Single-Stranded
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DNA-Binding Proteins
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Multiprotein Complexes
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Nuclear Proteins
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SMC4 protein, Xenopus
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X-ray Repair Cross Complementing Protein 1
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XCAP-E protein, Xenopus
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XRCC1 protein, human
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Xenopus Proteins
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Xrcc1 protein, mouse
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condensin complexes
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Poly(ADP-ribose) Polymerases
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Adenosine Triphosphatases