Abstract
Imaging studies implicate microtubule targeting of focal adhesions in focal adhesion disassembly, although the molecular mechanism is unknown. Here, we develop a model system of focal adhesion disassembly based on the finding that microtubule regrowth after nocodazole washout induces disassembly of focal adhesions, and that this disassembly occurs independently of Rho and Rac, but depends on focal adhesion kinase (FAK) and dynamin. During disassembly, dynamin interacts with FAK and colocalizes with focal adhesions. Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology
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Cell Adhesion / physiology*
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Cell Movement / physiology
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Dynamins / metabolism*
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Dynamins / ultrastructure
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Focal Adhesions / metabolism*
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Focal Adhesions / ultrastructure
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Green Fluorescent Proteins
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Integrins / metabolism
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Mice
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Microtubules / metabolism*
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Microtubules / ultrastructure
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NIH 3T3 Cells
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Nocodazole / pharmacology
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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rho GTP-Binding Proteins / metabolism
Substances
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Antineoplastic Agents
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Integrins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Protein-Tyrosine Kinases
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Ptk2 protein, mouse
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rho GTP-Binding Proteins
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Dynamins
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Nocodazole