Proteomic analysis revealed a novel synaptic proline-rich membrane protein (PRR7) associated with PSD-95 and NMDA receptor

Biochem Biophys Res Commun. 2005 Feb 4;327(1):183-91. doi: 10.1016/j.bbrc.2004.11.154.

Abstract

Proteomic analyses have revealed a novel synaptic proline-rich membrane protein: PRR7 (proline rich 7), in the postsynaptic density (PSD) fraction of rat forebrain. PRR7 is 269 amino acid residues long, and displays a unique architecture, composed of a very short N-terminal extracellular region, a single membrane spanning domain, and a cytoplasmic domain possessing a proline-rich sequence and a C-terminal type-1 PDZ binding motif. A fraction of PRR7 accumulates in spines along with synapse maturation, and colocalizes with PSD-95 in a punctate pattern in rat hippocampal neural cultures. Immunoprecipitation and GST pull-down assays demonstrated that PRR7 binds to the third PDZ domain of PSD-95. In addition, the NMDA receptor subunits, NR1 and NR2B, specifically co-immunoprecipitated with PRR7. These results suggest that PRR7 is involved in modulating neural activities via interactions with the NMDA receptor and PSD-95, and PSD core formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Antibody Affinity
  • Brain / cytology
  • Brain / metabolism
  • Cells, Cultured
  • Detergents / pharmacology
  • Disks Large Homolog 4 Protein
  • Gene Expression Profiling
  • Hippocampus / cytology
  • Hippocampus / metabolism
  • Humans
  • Immunoprecipitation
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / immunology
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding
  • Protein Structure, Tertiary
  • Protein Transport
  • Proteomics*
  • Rats
  • Rats, Wistar
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Sequence Alignment
  • Solubility / drug effects
  • Tissue Extracts

Substances

  • Antibodies
  • Detergents
  • Disks Large Homolog 4 Protein
  • Dlg4 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • NR1 NMDA receptor
  • NR2B NMDA receptor
  • Nerve Tissue Proteins
  • PRR7 protein, rat
  • Receptors, N-Methyl-D-Aspartate
  • Tissue Extracts
  • postsynaptic density proteins