Abstract
We have used antisense morpholino oligos to establish the developmental roles of three Xsox17 proteins in Xenopus development (Xsox17alpha(1), alpha(2) and beta). We show that their synthesis can be inhibited with modest amounts of oligo. The inhibition of each individually produces defects in late midgut development. Loss of activity of the Xsox17alpha proteins additionally inhibits hindgut formation, and inhibiting Xsox17alpha(1) disrupts foregut development with variable penetrance. When all Xsox17 activity is inhibited cell movements are halted during late gastrulation and the transcription of several endodermally expressed genes is reduced. Thus the Xsox17 proteins have redundant roles in early development of the endoderm and partly distinct roles during later organogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions
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Animals
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Cell Movement / genetics
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DNA-Binding Proteins / genetics
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Digestive System / embryology
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Digestive System Abnormalities
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Embryo, Nonmammalian
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Endoderm / physiology*
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Gene Expression Regulation, Developmental / drug effects
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Hepatocyte Nuclear Factor 1-beta
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High Mobility Group Proteins*
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In Vitro Techniques
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Larva
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Multigene Family
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Oligonucleotides, Antisense / pharmacology
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Phenotype
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Protein Biosynthesis
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Proteins / genetics*
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Proteins / metabolism
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SOXF Transcription Factors
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Transcription Factors / drug effects
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Transcription Factors / genetics
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Xenopus / embryology*
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Xenopus / genetics
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Xenopus Proteins / drug effects
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Xenopus Proteins / genetics*
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Xenopus Proteins / metabolism
Substances
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5' Untranslated Regions
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A2M protein, Xenopus
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DNA-Binding Proteins
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High Mobility Group Proteins
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Hnf1b protein, Xenopus
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Oligonucleotides, Antisense
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Proteins
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SOXF Transcription Factors
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Transcription Factors
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Xenopus Proteins
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sox17a protein, Xenopus
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sox17b.1 protein, Xenopus
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Hepatocyte Nuclear Factor 1-beta