Abstract
Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Blotting, Western
-
Cloning, Molecular
-
DNA, Recombinant
-
Epitopes / genetics*
-
Fungal Proteins / genetics
-
Fungal Proteins / metabolism
-
Genes, Fungal / genetics*
-
Genetic Markers
-
Genetic Vectors
-
Hemagglutinin Glycoproteins, Influenza Virus / genetics
-
Histidine / genetics
-
Humans
-
Integrases
-
Kanamycin Resistance
-
Membrane Glycoproteins*
-
Molecular Sequence Data
-
Plasmids / genetics*
-
Proto-Oncogene Proteins c-myc / genetics
-
Saccharomyces cerevisiae / genetics*
-
Transformation, Genetic
-
Viral Envelope Proteins / genetics
-
Viral Proteins*
Substances
-
DNA, Recombinant
-
Epitopes
-
Fungal Proteins
-
G protein, vesicular stomatitis virus
-
Genetic Markers
-
Hemagglutinin Glycoproteins, Influenza Virus
-
Membrane Glycoproteins
-
Proto-Oncogene Proteins c-myc
-
Viral Envelope Proteins
-
Viral Proteins
-
Histidine
-
Cre recombinase
-
Integrases
Associated data
-
GENBANK/AJ132965
-
GENBANK/AJ132966
-
GENBANK/AJ132967