A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

Gene. 2000 Apr 4;246(1-2):179-85. doi: 10.1016/s0378-1119(00)00083-4.

Abstract

Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cloning, Molecular
  • DNA, Recombinant
  • Epitopes / genetics*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genes, Fungal / genetics*
  • Genetic Markers
  • Genetic Vectors
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Histidine / genetics
  • Humans
  • Integrases
  • Kanamycin Resistance
  • Membrane Glycoproteins*
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Proto-Oncogene Proteins c-myc / genetics
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic
  • Viral Envelope Proteins / genetics
  • Viral Proteins*

Substances

  • DNA, Recombinant
  • Epitopes
  • Fungal Proteins
  • G protein, vesicular stomatitis virus
  • Genetic Markers
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins c-myc
  • Viral Envelope Proteins
  • Viral Proteins
  • Histidine
  • Cre recombinase
  • Integrases

Associated data

  • GENBANK/AJ132965
  • GENBANK/AJ132966
  • GENBANK/AJ132967