Abstract
The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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CCAAT-Binding Factor*
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Cell Cycle Proteins*
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Chromatin / metabolism
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal / genetics*
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Herpes Simplex Virus Protein Vmw65 / metabolism
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Nuclear Proteins*
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Nucleosomes / metabolism
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Promoter Regions, Genetic*
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Protein Kinases / metabolism
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation / genetics
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Yeasts / metabolism
Substances
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CCAAT-Binding Factor
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Cell Cycle Proteins
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Chromatin
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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HAP4 protein, S cerevisiae
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Herpes Simplex Virus Protein Vmw65
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Nuclear Proteins
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Nucleosomes
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Recombinant Proteins
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SWI5 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Protein Kinases