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The Proteomics Protocols Handbook pp 571–607Cite as

Protein Identification and Analysis Tools on the ExPASy Server

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Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Protein identification and analysis software performs a central role in the investigation of proteins from two-dimensional (2-D) gels and mass spectrometry. For protein identification, the user matches certain empirically acquired information against a protein database to define a protein as already known or as novel. For protein analysis, information in protein databases can be used to predict certain properties about a protein, which can be useful for its empirical investigation. The two processes are thus complementary. Although there are numerous programs available for those applications, we have developed a set of original tools with a few main goals in mind. Specifically, these are:

  1. 1.

    To utilize the extensive annotation available in the Swiss-Prot database (1) wherever possible, in particular the position-specific annotation in the Swiss-Prot feature tables to take into account posttranslational modifications and protein processing.

  2. 2.

    To develop tools specifically, but not exclusively, applicable to proteins prepared by twodimensional gel electrophoresis and peptide mass fingerprinting experiments.

  3. 3.

    To make all tools available on the World-Wide Web (WWW), and freely usable by the scientific community.

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Author information

Authors and Affiliations

  1. Swiss Institute of Bioinformatics, Geneva, Switzerland

    Elisabeth Gasteiger, Christine Hoogland, Alexandre Gattiker & S'everine Duvaud

  2. Proteome Systems Ltd., Sydney, Australia

    Marc R. Wilkins

  3. Swiss Institute of Bioinformatics, University and Geneva University Hospital, Geneva, Switzerland

    Ron D. Appel

  4. Swiss Institute of Bioinformatics, Geneva, Switzerland

    Amos Bairoch

Authors
  1. Elisabeth Gasteiger
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  2. Christine Hoogland
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  3. Alexandre Gattiker
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  4. S'everine Duvaud
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  5. Marc R. Wilkins
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  6. Ron D. Appel
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  7. Amos Bairoch
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 2005 Humana Press Inc., Totowa, NJ

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Gasteiger, E. et al. (2005). Protein Identification and Analysis Tools on the ExPASy Server. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:571

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  • DOI: https://doi.org/10.1385/1-59259-890-0:571

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-343-5

  • Online ISBN: 978-1-59259-890-8

  • eBook Packages: Springer Protocols

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