1887

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Volume 93, Issue 1

Viperin inhibits hepatitis C virus replication by interfering with binding of NS5A to host protein hVAP-33 Free

Abstract

Viperin is a type-I and -II interferon-inducible intracytoplasmic protein that mediates antiviral activity against several viruses. A previous study has reported that viperin could limit hepatitis C virus (HCV) replication . However, the underlying mechanism remains elusive. In the present study, we found that overexpression of viperin could inhibit HCV replication in a dose-dependent manner in both the replicon and HCVcc systems. Furthermore, through co-immunoprecipitation and laser confocal microscopic analysis, viperin was found to interact with the host protein hVAP-33. Mutagenesis analysis demonstrated that the anti-HCV activity of viperin was located to its C terminus, which was required for the interaction with the C-terminal domain of hVAP-33. Competitive co-immunoprecipitation analysis showed that viperin could interact competitively with hVAP-33, and could therefore interfere with its interactions with HCV NS5A. In summary, these findings suggest a novel mechanism by which viperin inhibits HCV replication, possibly through binding to host protein hVAP-33 and interfering with its interaction with NS5A.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-01-01
2024-04-16
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Viperin inhibits hepatitis C virus replication by interfering with binding of NS5A to host protein hVAP-33
J. Gen. Virol. 93, 83 (2012); https://doi.org/10.1099/vir.0.033860-0
/content/journal/jgv/10.1099/vir.0.033860-0
/content/journal/jgv/10.1099/vir.0.033860-0
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