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Volume 157, Issue 10

Characterization of the role of the RadS/RadR two-component system in the radiation resistance of Free

Abstract

shows extraordinary tolerance to DNA damage, and exhibits differential gene expression and protein recycling. A putative response regulator, the DRB0091 (RadR) ORF, was identified from a pool of DNA-binding proteins induced in response to gamma radiation in this bacterium. is located upstream of , which encodes a putative sensor histidine kinase (RadS) on the megaplasmid. Deletion of these genes both individually and together resulted in hypersensitivity to DNA-damaging agents and a delayed or altered double-strand break repair. A Δ double mutant and a Δ single mutant showed nearly identical responses to gamma radiation and UVC. Wild-type RadR and RadS complemented the corresponding mutant strains, but also exhibited significant cross-complementation, albeit at lower doses of gamma radiation. The transcript was not detected in the Δ mutant, suggesting the existence of a operon. Recombinant RadS was autophosphorylated and could catalyse the transfer of γ phosphate from ATP to RadR . These results indicated the functional interaction of RadS and RadR, and suggested a role for the RadS/RadR two-component system in the radiation resistance of this bacterium.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Bhabha Atomic Research Centre
© 2011 SGM
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2011-10-01
2024-04-19
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Characterization of the role of the RadS/RadR two-component system in the radiation resistance of Deinococcus radiodurans
Microbiology 157, 2974 (2011); https://doi.org/10.1099/mic.0.049361-0
/content/journal/micro/10.1099/mic.0.049361-0
/content/journal/micro/10.1099/mic.0.049361-0
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