Issue 19, 2021
From the journal:

Chemical Communications

cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins†

Taishi Kondo, ORCID logo a   Minori Eguchi, ORCID logo a   Seita Kito,a   Tomoshige Fujino, ORCID logo a   Gosuke Hayashi ORCID logo ab  and  Hiroshi Murakami ORCID logo *ac  

* Corresponding authors

a Graduate School of Engineering, Nagoya University, Nagoya, Japan
E-mail: murah@chembio.nagoya-u.ac.jp

b Japan Science and Technology Agency (JST), PRESTO, Kawaguchi, Japan

c Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan

Abstract

We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.

Graphical abstract: cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins

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Article information

DOI
https://doi.org/10.1039/D0CC07541H
Article type
Communication
Submitted
17 Nov 2020
Accepted
02 Feb 2021
First published
02 Feb 2021

Chem. Commun., 2021,57, 2416-2419

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cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins

T. Kondo, M. Eguchi, S. Kito, T. Fujino, G. Hayashi and H. Murakami, Chem. Commun., 2021, 57, 2416 DOI: 10.1039/D0CC07541H

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