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Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer

Oncogene volume 20, pages 4402–4408 (2001)Cite this article

Abstract

We report the identification of a novel human tumor associated gene, CDCP1 (Cub Domain Containing Protein), which was identified using representational difference analysis and cDNA chip technology. The gene consists of eight exons, the upstream region of which neither contains a TATA- nor a CCAAT-box. However, a CpG island is located around the transcription start, which is found in approximately 60% of known genes. The CDCP1 gene was mapped to chromosome 3p21-p23 by fluorescence in situ hybridization. For expression profiling real time quantitative RT–PCR was performed using cell lines and laser capture microdissected colon cancer biopsies. CDCP1 mRNA is approximately 6 kb and highly overexpressed in human colon cancer and lung cancer. CDCP1 represents a putative transmembrane protein, containing three CUB domains in the extracellular part most likely involved in cell adhesion or interacting with the extracellular matrix.

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Acknowledgements

We thank H Dauwa-Stummer and A Gupta for excellent technical assistance and R Varecka for DNA sequencing. We would like to acknowledge K Zatloukal (Dept. of Pathology, University of Graz) for generously providing the colon cancer patient samples and we want to thank L Wightman for critically reading the manuscript. Last but not least we want to thank KK Wilgenbus, G Adolf and M Baccarini for helpful discussions.

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Authors and Affiliations

  1. Research and Development, Dept. of Exploratory Research, Boehringer Ingelheim Austria, Vienna, 1121, Austria

    Marwa Scherl-Mostageer, Wolfgang Sommergruber, Roger Abseher, Rudolf Hauptmann & Norbert Schweifer

  2. CCRI, St. Anna Kinderspital, Vienna, 1080, Austria

    Peter Ambros

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  1. Marwa Scherl-Mostageer
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  2. Wolfgang Sommergruber
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  3. Roger Abseher
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  4. Rudolf Hauptmann
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Correspondence to Norbert Schweifer.

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Scherl-Mostageer, M., Sommergruber, W., Abseher, R. et al. Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer. Oncogene 20, 4402–4408 (2001). https://doi.org/10.1038/sj.onc.1204566

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