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Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis

Oncogene volume 29, pages 1103–1113 (2010)Cite this article

Abstract

Recent studies suggest that some serous ovarian carcinomas (SOCs) arise from the fallopian tube (FT) epithelium rather than the ovarian surface epithelium. This hypothesis places emphasis on the FT secretory epithelial cell as a cell-of-origin. Herein, we report the development of a novel ex vivo primary human FT epithelium culture system that faithfully recapitulates the in vivo epithelium, as shown by morphological, ultrastructural and immunophenotypic analyses. Mass spectrometry-based proteomics reveal that these cultures secrete proteins previously identified as biomarkers for ovarian cancer. We also use this culture system to study the response of the FT epithelium to genotoxic stress and find that the secretory cells exhibit a distinct response to DNA damage when compared with neighboring ciliated cells. The secretory cells show a limited ability to resolve the damage over time, potentially leaving them more susceptible to accumulation of additional mutagenic injury. This divergent response is confirmed with in situ studies using tissue samples, further supporting the use of this ex vivo culture system to investigate FT epithelial pathobiology. We anticipate that this novel culture system will facilitate the study of SOC pathogenesis, and propose that similar culture systems could be developed for other organ site-specific epithelia.

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Acknowledgements

We thank Phil Karp, Thomas Moninger and Drs Paola Vermeer and Joseph Zabner (University of Iowa) for their enthusiastic support and assistance in establishing the FT ex vivo culture system, Drs Steve Cannistra, Glenn Dranoff and David Livingston for thoughtful suggestions and comments on the paper and Drs Tom Benjamin and Dawei Li for the Sall2 antibody. Special thanks goes to the faculty, technicians, residents and fellows of the division of Women's and Perinatal Pathology in the Department of Pathology at the Brigham and Women's Hospital, Boston, MA, for the allocation of tissues. This work was supported by the National Cancer Institute [P50 CA105009, K08 CA108748 and R21 CA124688], Ovarian Cancer Research Fund (Individual Investigator Award and Program Project Development Award), Phi Beta Psi Sorority Charitable Trust, Fannie E. Ripple Foundation, Robert and Deborah First Fund, Randi and Joel Cutler Ovarian Cancer Research Fund, the Columbia Hospital for Women Research Foundation, Marsha Rivkin Foundation Scientific Scholar Award, AACR-George and Patricia Sehl Fellowship for Cancer Genetics Research and the American Physicians Fellowship for Medicine in Israel- Claire and Emmanuel G Rosenblatt Foundation Grant.

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Authors and Affiliations

  1. Department of Medical Oncology, Center of Molecular Oncologic Pathology, Dana-Farber Cancer Institute, Boston, MA, USA

    K Levanon, V Ng, H Y Piao & R Drapkin

  2. Department of Cancer Biology, Center of Proteomics, Dana-Farber Cancer Institute, Boston, MA, USA

    Yi Zhang & J A Marto

  3. Department of Pathology, Division of Women's and Perinatal Pathology, Brigham and Women's Hospital, Boston, MA, USA

    M C Chang, M H Roh, D W Kindelberger, M S Hirsch, C P Crum & R Drapkin

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  1. K Levanon
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  2. V Ng
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  3. H Y Piao
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Correspondence to R Drapkin.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Levanon, K., Ng, V., Piao, H. et al. Primary ex vivo cultures of human fallopian tube epithelium as a model for serous ovarian carcinogenesis. Oncogene 29, 1103–1113 (2010). https://doi.org/10.1038/onc.2009.402

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