Abstract
The histone chaperone SET is required for transcription of chromatin templates by RNA polymerase Pol II (Pol II) in vitro. Here we uncover a positive role for SET in dislodging DEK and PARP1, which restrict access to chromatin in the absence of SET and the PARP1 substrate NAD+. SET binds chromatin, dissociating DEK and PARP1 to allow transcription in the absence of NAD+. In the absence of SET, depletion of DEK restores chromatin accessibility to endonuclease but does not permit Mediator recruitment or transcription. In the presence of NAD+, PARP1 poly(ADP-ribosyl)ates and evicts DEK (and itself) from chromatin to permit Mediator loading and transcription independent of SET. An artificial DEK variant resistant to SET and PARP1 represses transcription, indicating a requirement for DEK removal. Therefore, SET, DEK and PARP1 constitute a network governing access to chromatin by the transcription machinery.
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Acknowledgements
We thank S. Larochelle and J. Lis (Cornell University) for critical review of the manuscript and for many helpful discussions; W. Lee Kraus (Cornell University) for helpful comments and suggestions, for PARP1 and Gal4-VP16 expression vectors and pGEIO plasmid, and for material support during the revision process; H. Erdjument-Bromage and P. Tempst for mass spectrometric identification of DEK, SET and PARP1; T. Ito (Nagasaki University) for core histone–specific antibodies; J. Kadonaga (University of California, San Diego) for NAP1, ISWI and Acf1 baculoviruses; M. Ptashne for Gal4 DNA-binding domain–specific antibodies; and R. Roeder (Rockefeller University) for MED30-specific antibodies. HeLa cells were provided by the National Cell Culture Center (US). This work was supported in part by US National Institutes of Health grant DK45460 to R.P.F.
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M.J.G. designed and executed experiments and prepared the manuscript; R.P.F. prepared the manuscript.
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Supplementary information
Supplementary Fig. 1
Time course of restriction endonuclease accessibility of chromatin in the presence of SET and/or the PC1 fraction. (PDF 78 kb)
Supplementary Fig. 2
Removal of DEK is not sufficient to restore chromatin transcription of PIC assembly in the absence of SET. (PDF 557 kb)
Supplementary Fig. 3
GST or thrombin does not affect chromatin transcription. (PDF 390 kb)
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Gamble, M., Fisher, R. SET and PARP1 remove DEK from chromatin to permit access by the transcription machinery. Nat Struct Mol Biol 14, 548–555 (2007). https://doi.org/10.1038/nsmb1248
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DOI: https://doi.org/10.1038/nsmb1248
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