Abstract
Tristetraprolin (TTP) is an RNA-binding protein that controls the inflammatory response by limiting the expression of several proinflammatory cytokines. TTP post-transcriptionally represses gene expression by interacting with AU-rich elements (AREs) in 3′ untranslated regions of target mRNAs and subsequently engenders their deadenylation and decay. TTP accomplishes these tasks, at least in part, by recruiting the multisubunit CCR4–NOT deadenylase complex to the mRNA. Here we identify an evolutionarily conserved C-terminal motif in human TTP that directly binds a central domain of CNOT1, a core subunit of the CCR4–NOT complex. A high-resolution crystal structure of the TTP–CNOT1 complex was determined, providing the first structural insight, to our knowledge, into an ARE-binding protein bound to the CCR4–NOT complex. Mutations at the CNOT1-TTP interface impair TTP-mediated deadenylation, demonstrating the significance of this interaction in TTP-mediated gene silencing.
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Acknowledgements
This work was supported by Canadian Institutes of Health Research grants to N. Sonenberg (MOP-93607) and to B.N. (MOP-82929). N. Sonenberg was also supported by a Howard Hughes Medical Institute Senior International Scholarship. P.J.B. was supported by the Intramural Research Program of the US National Institutes of Health, National Institute of Environmental Health Sciences. N. Siddiqui is supported by a fellowship from the Cole Foundation.
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M.R.F., F.F., C.R. and N. Siddiqui designed experiments. M.R.F. and C.R. performed binding assays and in vitro deadenylation assays. N. Siddiqui performed ITC experiments. F.F. crystallized the CNOT1–TTP complex, and B.N. and F.F. determined its structure. W.S.L. and P.J.B. performed in vivo stability assays. A.K. provided technical support. M.R.F., F.F., B.N. and N. Sonenberg wrote the manuscript.
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Fabian, M., Frank, F., Rouya, C. et al. Structural basis for the recruitment of the human CCR4–NOT deadenylase complex by tristetraprolin. Nat Struct Mol Biol 20, 735–739 (2013). https://doi.org/10.1038/nsmb.2572
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DOI: https://doi.org/10.1038/nsmb.2572
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