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References
Henderson, R. et al. Structure 20, 205–214 (2012).
Saxton, W.O. & Baumeister, W. J. Microsc. 127, 127–138 (1982).
Stewart, A. & Grigorieff, N. Ultramicroscopy 102, 67–84 (2004).
Scheres, S.H. J. Mol. Biol. 415, 406–418 (2012).
Henderson, R. et al. J. Mol. Biol. 413, 1028–1046 (2011).
Scheres, S.H. et al. Nat. Protoc. 3, 977–990 (2008).
Böttcher, B., Wynne, S.A. & Crowther, R.A. Nature 386, 88–91 (1997).
Rosenthal, P.B. & Henderson, R. J. Mol. Biol. 333, 721–745 (2003).
Acknowledgements
We are grateful to T. Crowther and R. Henderson for helpful discussions and to J. Grimmett for help with computing. T. Crowther provided hepatitis B data, and the National Center for Macromolecular Imaging, which is funded by US National Institutes of Health grant P41RR02250, provided GroEL data. This work was funded by the UK Medical Research Council through grant MC_UP_A025_1013 to S.H.W.S.
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Scheres, S., Chen, S. Prevention of overfitting in cryo-EM structure determination. Nat Methods 9, 853–854 (2012). https://doi.org/10.1038/nmeth.2115
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DOI: https://doi.org/10.1038/nmeth.2115
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