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Improved Mos1-mediated transgenesis in C. elegans

Nature Methods volume 9, pages 117–118 (2012)Cite this article

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To the Editor:

The ability to add or delete genes to the genome of genetic model organisms is essential. Previously, we had developed methods based on the Mos1 transposon1 to make targeted transgene insertions (Mos1-mediated single-copy transgene insertions; MosSCI2) and targeted deletions (Mos1-mediated deletions; MosDEL3) in Caenorhabditis elegans, the latter published in Nature Methods. Here we present new reagents that improve the efficiency, facilitate the selection for transgenic strains and expand the set of MosSCI insertion sites (Supplementary Table 1).

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Figure 1: Improvements to Mos1-based genome manipulation.

References

  1. Robert, V. & Bessereau, J.L. EMBO J. 26, 170–183 (2007).

    Article  CAS  Google Scholar 

  2. Frøkjær-Jensen, C. et al. Nat. Genet. 40, 1375–1383 (2008).

    Article  Google Scholar 

  3. Frøkjær-Jensen, C. et al. Nat. Methods 7, 451–453 (2010).

    Article  Google Scholar 

  4. Seidel, H.S. et al. PLoS Biol. 9, e1001115 (2011).

    Article  CAS  Google Scholar 

  5. Giordano-Santini, R. et al. Nat. Methods 7, 721–723 (2010).

    Article  CAS  Google Scholar 

  6. Semple, J.I., Garcia-Verdugo, R. & Lehner, B. Nat. Methods 7, 725–727 (2010).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

C.F.-J. was funded by postdoctoral stipends from the Lundbeck foundation and the Carlsberg foundation. M.A. was supported by a Helen Hay Whitney Postdoctoral Fellowship and by the US National Institutes of Health (K99MH082109). This research was funded by US National Institutes of Health (1R01GM095817) and Howard Hughes Medical Institute.

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  1. Michael Ailion

    Present address: Present address: Department of Biochemistry, University of Washington, Seattle, Washington, USA.,

Authors and Affiliations

  1. Department of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA

    Christian Frøkjær-Jensen, M Wayne Davis, Michael Ailion & Erik M Jorgensen

  2. Department of Biomedical Sciences and Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark

    Christian Frøkjær-Jensen

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  1. Christian Frøkjær-Jensen
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  2. M Wayne Davis
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  3. Michael Ailion
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  4. Erik M Jorgensen
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Correspondence to Erik M Jorgensen.

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Competing interests

E.M.J. is an author of a patent covering techniques described in this paper (US patent 7,196,244 and European patent pending).

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Supplementary Figures 1–5, Supplementary Table 1 and Supplementary Methods (PDF 3070 kb)

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Frøkjær-Jensen, C., Davis, M., Ailion, M. et al. Improved Mos1-mediated transgenesis in C. elegans. Nat Methods 9, 117–118 (2012). https://doi.org/10.1038/nmeth.1865

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